Hauschildt S, Hirt W, Bessler W
Institut für Immunbiologie, Universität Freiburg, FRG.
FEBS Lett. 1988 Mar 28;230(1-2):121-4. doi: 10.1016/0014-5793(88)80654-9.
Stimulation of murine bone marrow derived macrophages with NaF, prelabeled with [1-14C]oleate and [3H]inositol, increased the production of inositol phosphates and the release of 1,2-[14C]diacylglycerol (DAG). Moreover, NaF also induced activation of protein kinase C. These results indicate that bone marrow derived macrophages exhibit a phosphatidyl-4,5-bisphosphate phospholipase C activity, sensitive to NaF, which might be modulated by G-proteins. Activation of protein kinase C could have been mediated by NaF-induced release of DAG.
用[1-14C]油酸酯和[3H]肌醇预标记的氟化钠刺激小鼠骨髓来源的巨噬细胞，可增加肌醇磷酸的产生以及1,2-[14C]二酰甘油（DAG）的释放。此外，氟化钠还可诱导蛋白激酶C的激活。这些结果表明，骨髓来源的巨噬细胞表现出对氟化钠敏感的磷脂酰-4,5-二磷酸磷脂酶C活性，该活性可能受G蛋白调节。蛋白激酶C的激活可能是由氟化钠诱导的DAG释放介导的。
