School of Molecular Sciences & The ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley, Perth, 6009, Australia.
New Phytol. 2018 May;218(3):923-928. doi: 10.1111/nph.14511. Epub 2017 Mar 21.
Contents Summary 923 I. Introduction 923 II. Plant AEPs with macrocyclizing ability 924 III. Mechanism of macrocyclization by AEPs 925 IV. Conclusions 927 Acknowledgements 927 References 927 SUMMARY: Plant asparaginyl endopeptidases (AEPs) are important for the post-translational processing of seed storage proteins via cleavage of precursor proteins. Some AEPs also function as peptide bond-makers during the biosynthesis of several unrelated classes of cyclic peptides, namely the kalata-type cyclic peptides, PawS-Derived Peptides and cyclic knottins. These three families of gene-encoded peptides have different evolutionary origins, but all have recruited AEPs for their maturation. In the last few years, the field has advanced rapidly, with the biochemical characterization of three plant AEPs capable of peptide macrocyclization, and insights have been gained from the first AEP crystal structures, albeit mammalian ones. Although the biochemical studies have improved our understanding of the mechanism of action, the focus now is to understand what changes in AEP sequence and structure enable some plant AEPs to perform macrocyclization reactions.
内容摘要 923 I. 引言 923 II. 具有大环化能力的植物天冬酰胺内肽酶 924 III. AEP 进行大环化的机制 925 IV. 结论 927 致谢 927 参考文献 927 摘要：植物天冬酰胺内肽酶（AEPs）在通过切割前体蛋白对种子贮藏蛋白进行翻译后加工中很重要。一些 AEPs 还在几种不相关的环肽（即 kalata 型环肽、PawS 衍生肽和环 knotin）的生物合成中作为肽键形成酶发挥作用。这三个基因编码的肽家族具有不同的进化起源，但都招募了 AEPs 来实现其成熟。在过去的几年中，该领域取得了快速进展，已有三种能够进行肽大环化的植物 AEP 的生化特征得到了描述，并从第一个 AEP 晶体结构中获得了一些见解，尽管这些晶体结构是哺乳动物的。尽管生化研究提高了我们对作用机制的理解，但现在的重点是了解 AEP 序列和结构的哪些变化使一些植物 AEP 能够进行大环化反应。
