Asani Swati C, Umrani Rinku D, Paknikar Kishore M
Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, Maharashtra, India.
Nanomedicine (Lond). 2017 Apr;12(7):745-759. doi: 10.2217/nnm-2016-0426. Epub 2017 Mar 21.
To study the effects of zinc oxide nanoparticles (ZON) on oxidative stress-mediated pancreatic β-cell death.
Cellular uptake of ZON, effects on antioxidant factors and apoptosis were studied.
ZON get internalized by endocytosis and increase intracellular zinc ion levels. ZON treatment (30 and 100 μg/ml) to RIN5f cells resulted in cytotoxicity, oxidative stress and apoptosis. ZON (1, 3, 10 μg/ml, subcytotoxic concentrations) increased super oxide dismutase activity and levels of reduced glutathione in RIN5f cells. Furthermore, ZON (subcytotoxic concentrations) protected RIN5f cells from HO-induced oxidative stress as evidenced by reduced reactive oxygen species levels; increased super oxide dismutase activity and glutathione levels; and reduced apoptotic death.
ZON (subcytotoxic concentrations) protect pancreatic β cells from oxidative-stress-mediated cell death.
研究氧化锌纳米颗粒（ZON）对氧化应激介导的胰腺β细胞死亡的影响。
研究了ZON的细胞摄取、对抗氧化因子的影响及细胞凋亡情况。
ZON通过内吞作用进入细胞并增加细胞内锌离子水平。用ZON（30和100μg/ml）处理RIN5f细胞会导致细胞毒性、氧化应激和细胞凋亡。ZON（1、3、10μg/ml，亚细胞毒性浓度）可增加RIN5f细胞中超氧化物歧化酶活性和还原型谷胱甘肽水平。此外，ZON（亚细胞毒性浓度）可保护RIN5f细胞免受HO诱导的氧化应激，表现为活性氧水平降低；超氧化物歧化酶活性和谷胱甘肽水平增加；凋亡死亡减少。
ZON（亚细胞毒性浓度）可保护胰腺β细胞免受氧化应激介导的细胞死亡。
