Matthews T J, Langlois A J, Robey W G, Chang N T, Gallo R C, Fischinger P J, Bolognesi D P
Department of Surgery, Duke University Medical School, Durham, North Carolina 27710.
Haematol Blood Transfus. 1987;31:414-22. doi: 10.1007/978-3-642-72624-8_89.
By analogy to other retroviruses, the major envelope glycoprotein - gp120 - of HTLV-III/LAV is a probable target for neutralizing antibody. This antigen has been purified from H9 cells chronically infected with the HTLV-IIIB prototype strain. Several goats immunized with the gp120 produced antibodies that neutralized infection of H9 by the homologous virus isolate. These same sera failed to neutralize the divergent HTLV-IIIRF isolate. Individuals infected with HTLV-III/LAV commonly develop antibodies to gp120 which could be isolated using the gp120 antigen coupled to an immunoadsorbent resin. The antibody fraction that bound tightly to such a resin was found to neutralize the IIIB but not the RF isolate in a fashion similar to that of the goat anti-gp120 sera. However, the nonbinding fraction (effluent) from the resin also contained neutralizing activity which was able to block infection by both virus isolates with similar efficacy. Human antibodies to the other virus envelope gene product, the transmembrane gp41, were also affinity-purified utilizing the recombinant peptide 121, but they failed to influence infection by either virus isolate.
与其他逆转录病毒类似，HTLV-III/LAV的主要包膜糖蛋白——gp120——可能是中和抗体的作用靶点。该抗原已从长期感染HTLV-IIIB原型毒株的H9细胞中纯化出来。几只用gp120免疫的山羊产生了能中和同源病毒分离株对H9细胞感染的抗体。这些血清无法中和不同的HTLV-IIIRF分离株。感染HTLV-III/LAV的个体通常会产生针对gp120的抗体，这些抗体可用与免疫吸附树脂偶联的gp120抗原分离出来。发现与这种树脂紧密结合的抗体部分能够中和IIIB分离株，但不能中和RF分离株，其方式与山羊抗gp120血清类似。然而，树脂的未结合部分（流出液）也含有中和活性，能够以相似的效力阻断两种病毒分离株的感染。针对另一种病毒包膜基因产物跨膜gp41的人源抗体也利用重组肽121进行了亲和纯化，但它们未能影响任何一种病毒分离株的感染。
