Venkataravanappa V, Lakshminarayana Reddy C N, Krishna Reddy M
Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore, 560089, India.
Division of Crop Protection, Indian Institute Vegetable Research, Varanasi, 221305, Uttar Pradesh, India.
3 Biotech. 2013 Dec;3(6):461-470. doi: 10.1007/s13205-012-0107-z. Epub 2012 Dec 20.
The leaf sample from okra plants showing the yellow vein mosaic disease symptoms was collected in Karnataka state, India. The genome of the virus was amplified, cloned and sequenced. Sequence analysis revealed that the viral genome (GU112065) is 2,741 bp in length and genome is similar to that of monopartite begomoviruses originating from the Old World, with seven conserved ORFs. Further nucleotide (nts) sequence comparisons showed that the genome has the highest sequence identities of 96.1 % with Bhendi yellow vein mosaic virus (BYVMV) (GU112057) and 89.7 % with okra yellow vein mosaic virus (OYVMV) (AJ002451) infecting okra in India and Indian subcontinent. These results suggested that the isolate is a new strain of BYVMV. To identify the resistance source to BYVMV, the okra genotypes were screened under both artificial and natural conditions. None of the genotypes showed immunity to the disease. However, the genotypes Nun 1145 and Nun 1144 showed moderate resistance and genotypes M10, Nun 1142, Nun 1140 showed moderately susceptible reactions under both glass house and field conditions. Further, dot-blot hybridization using nonradioactive (digoxigenin) DNA probe showed that the virus was also detected in the symptomless plants.
取自印度卡纳塔克邦表现出黄脉花叶病症状的秋葵植株的叶片样本。对该病毒的基因组进行了扩增、克隆和测序。序列分析表明，该病毒基因组（GU112065）长度为2741 bp，与源自旧大陆的单分体双生病毒基因组相似，有7个保守的开放阅读框。进一步的核苷酸（nts）序列比较显示，该基因组与感染印度和印度次大陆秋葵的本迪黄脉花叶病毒（BYVMV）（GU112057）的序列同一性最高，为96.1%，与秋葵黄脉花叶病毒（OYVMV）（AJ002451）的序列同一性为89.7%。这些结果表明该分离株是BYVMV的一个新毒株。为了鉴定对BYVMV的抗性来源，在人工和自然条件下对秋葵基因型进行了筛选。没有一个基因型对该病表现出免疫性。然而，基因型Nun 1145和Nun 1144表现出中度抗性，基因型M10、Nun 1142、Nun 1140在温室和田间条件下均表现出中度敏感反应。此外，使用非放射性（地高辛）DNA探针的斑点杂交显示，在无症状植株中也检测到了该病毒。
