Zhang Chunyan, Sun Wen, Tan Meifang, Dong Mengmeng, Liu Wanquan, Gao Ting, Li Lu, Xu Zhuofei, Zhou Rui
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
Veterinary Medicine Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences Nanchang, China.
Front Cell Infect Microbiol. 2017 Mar 7;7:66. doi: 10.3389/fcimb.2017.00066. eCollection 2017.
Like eukaryotes, bacteria express one or more serine/threonine kinases (STKs) that initiate diverse signaling networks. The STK from is encoded by a single-copy gene, which is crucial in stress response and virulence. To further understand the regulatory mechanism of STK in , a deletion strain (Δ) and its complementary strain (CΔ) were constructed to systematically decode STK characteristics by applying whole transcriptome RNA sequencing (RNA-Seq) and phosphoproteomic analysis. Numerous genes were differentially expressed in Δ compared with the wild-type parental strain SC-19, including 320 up-regulated and 219 down-regulated genes. Particularly, 32 virulence-associated genes (VAGs) were significantly down-regulated in Δ. Seven metabolic pathways relevant to bacterial central metabolism and translation are significantly repressed in Δ. Phosphoproteomic analysis further identified 12 phosphoproteins that exhibit differential phosphorylation in Δ. These proteins are associated with cell growth and division, glycolysis, and translation. Consistently, phenotypic assays confirmed that the Δ strain displayed deficient growth and attenuated pathogenicity. Thus, STK is a central regulator that plays an important role in cell growth and division, as well as metabolism.
与真核生物一样，细菌表达一种或多种启动不同信号网络的丝氨酸/苏氨酸激酶（STK）。[细菌名称]的STK由一个单拷贝基因编码，该基因在应激反应和毒力中至关重要。为了进一步了解[细菌名称]中STK的调控机制，构建了一个[基因名称]缺失菌株（Δ）及其互补菌株（CΔ），通过应用全转录组RNA测序（RNA-Seq）和磷酸化蛋白质组分析来系统地解码STK的特征。与野生型亲本菌株SC-19相比，Δ中有许多基因差异表达，包括320个上调基因和219个下调基因。特别地，32个毒力相关基因（VAGs）在Δ中显著下调。与细菌中心代谢和翻译相关的七条代谢途径在Δ中受到显著抑制。磷酸化蛋白质组分析进一步鉴定出12种在Δ中表现出磷酸化差异的磷酸化蛋白质。这些蛋白质与细胞生长和分裂、糖酵解及翻译有关。一致地，表型分析证实Δ菌株生长缺陷且致病性减弱。因此，STK是一种核心调节因子，在细胞生长和分裂以及[细菌名称]代谢中起重要作用。
