Lixia Xu, Hongmei Xu, Xiaoying Zhu, Limei Sun
Dept. of Stomatology, The General Hospital of Armed Police Force, Beijing 100039, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2016 Oct 1;34(5):516-520. doi: 10.7518/hxkq.2016.05.017.
Microstructural changes in dentin carious lesions were investigated using a 3D laser scanning microscope, which has a morphological theoretical foundation in the further study of clinical caries disease prevention and treatments.
Six fresh extracted caries molars were prepared into cross-section specimens. The sections were examined by 3D and laser measuring morphology.
Zones were identified in the lesions on the basis of their optical appearance. Two zones were identified in the lesions on the basis of their laser appearance. The microstructure showed that the tubular was partly closed in transparent dentin; peritubular and intertubular dentin were reduced in the zone of demineralization; peritubular and intertubular dentin were damaged and fused; a beaded sample and oval lesions formed in the zone of bacterial invasion; and abnormal dentin structure was present in the zone of destruction on the basis of their laser appearance. Four zones were iden-tified in the lesions according to their colors, as determined from their 3D appearance.
3D laser scanning micros-cope may be a powerful, accessible, and non-destructive technique, as it identified the lesion and tubular zones, as well as peritubular and intertubular dentin in the four zones' lesions. The microstructure of dentin caries lesions may have significant merit in the evaluation of clinical prevention and treatment.
使用三维激光扫描显微镜研究牙本质龋损的微观结构变化，这为进一步研究临床龋病的预防和治疗提供了形态学理论基础。
将六颗新鲜拔除的龋磨牙制成横断面标本。通过三维激光测量形态对切片进行检查。
根据病变的光学外观确定了病变区域。根据病变的激光外观确定了两个区域。微观结构显示，在透明牙本质中，牙本质小管部分封闭；在脱矿区域，管周牙本质和管间牙本质减少；管周牙本质和管间牙本质受损并融合；在细菌侵入区域形成串珠状样本和椭圆形病变；根据病变的激光外观，在破坏区域存在异常牙本质结构。根据病变的三维外观所确定的颜色，在病变中识别出四个区域。
三维激光扫描显微镜可能是一种强大、易用且无损的技术，因为它能识别病变和小管区域，以及四个区域病变中的管周牙本质和管间牙本质。牙本质龋损的微观结构在临床预防和治疗评估中可能具有重要价值。
