Irarrázaval Sebastián, Allard Claudio, Campodónico Juan, Pérez Druso, Strobel Pablo, Vásquez Luis, Urquiaga Inés, Echeverría Guadalupe, Leighton Federico
1 Department of Orthopedic Surgery, School of Medicine , Pontificia Universidad Católica de Chile, Santiago, Chile .
2 Center for Molecular Nutrition and Chronic Diseases, School of Medicine, Pontificia Universidad Católica de Chile , Santiago, Chile .
High Alt Med Biol. 2017 Jun;18(2):128-134. doi: 10.1089/ham.2016.0119. Epub 2017 Mar 22.
Irarrázaval, Sebastián, Claudio Allard, Juan Campodónico, Druso Pérez, Pablo Strobel, Luis Vásquez, Inés Urquiaga, Guadalupe Echeverría, and Federico Leighton. Oxidative stress in acute hypobaric hypoxia. High Alt Med Biol. 18:128-134, 2017.-The effects of acute hypobaric hypoxia endured by mountaineers were studied, specifically as evidenced by acute mountain sickness (AMS) and oxidative stress damage. Ten male volunteers were exposed to acute hypobaric hypoxia, and AMS was evaluated through arterial oxygen saturation (SaO), cardiac rate, and the Lake Louise Score (LLS). Oxidative stress was determined through blood profile tests performed 24 hours before and after high-altitude exposure, assessing the oxidative damage and antioxidant profiles. Dietary habits were assessed using the Chilean Mediterranean Diet Index. During ascent (i.e., first 8 hours), all volunteers presented AMS (LLS ≥3 points), as manifested by a median LLS increment of four points, a 15 bpm cardiac rate, and 17% decrease in SaO. Additionally, plasma lipid oxidative damage increased after the expedition, as evaluated through malondialdehyde, which was directly correlated with the LLS (R = 0.720, p = 0.003) and inversely correlated with SaO (R = 0.436; p = 0.035) at a high altitude. Preascent carbonyl levels were inversely correlated to SaO (R = 0.490; p = 0.008) and directly correlated to cardiac rate (R = 0.225, p = 0.016) at a high altitude. Moreover, dietary habits were inversely correlated with increased carbonyls during the expedition (R = 0.436; p = 0.047). In conclusion, acute hypobaric hypoxia induced AMS and an increment in oxidative stress markers 24 hours after altitude exposure in the volunteers. Furthermore, oxidative stress damage was related to AMS severity. Finally, volunteers with closer adherence to a Mediterranean diet presented a lower increase in oxidative damage during ascent, reflecting the potential preventive role of diet against AMS.
伊拉拉扎瓦尔、塞巴斯蒂安、克劳迪奥·阿拉德、胡安·坎波多尼科、德鲁索·佩雷斯、巴勃罗·斯特罗贝尔、路易斯·巴斯克斯、伊内斯·乌尔基阿加、瓜达卢佩·埃切维里亚和费德里科·莱顿。急性低压缺氧中的氧化应激。《高原医学与生物学》。2017年；18:128 - 134。——研究了登山者所经历的急性低压缺氧的影响，具体表现为急性高原病（AMS）和氧化应激损伤。10名男性志愿者暴露于急性低压缺氧环境中，通过动脉血氧饱和度（SaO）、心率和路易斯湖评分（LLS）评估AMS。通过在高原暴露前后24小时进行的血液检测来确定氧化应激，评估氧化损伤和抗氧化指标。使用智利地中海饮食指数评估饮食习惯。在登山过程中（即最初8小时），所有志愿者均出现AMS（LLS≥3分），表现为LLS中位数增加4分、心率增加15次/分钟以及SaO降低17%。此外，通过丙二醛评估发现，探险后血浆脂质氧化损伤增加，其与LLS直接相关（R = 0.720，p = 0.003），在高原时与SaO呈负相关（R = 0.436；p = 0.035）。登山前羰基水平在高原时与SaO呈负相关（R = 0.490；p = 0.008），与心率呈正相关（R = 0.225，p = 0.016）。此外，饮食习惯与探险期间羰基增加呈负相关（R = 0.436；p = 0.047）。总之，急性低压缺氧在志愿者高原暴露24小时后诱发了AMS并使氧化应激标志物增加。此外，氧化应激损伤与AMS严重程度相关。最后，更严格遵循地中海饮食的志愿者在登山过程中氧化损伤增加较少，这反映了饮食对AMS的潜在预防作用。
