超氧自由基生成系统对中国仓鼠卵巢细胞DNA双链稳定性的急性影响。通过改良荧光法测定。

Acute effects of a superoxide radical-generating system on DNA double-strand stability in Chinese hamster ovary cells. Determination by a modified fluorometric procedure.

作者信息

Hall A H, Eanes R Z, Waymack P P, Patterson R M

机构信息

Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333.

出版信息

Mutat Res. 1988 Mar;198(1):161-8. doi: 10.1016/0027-5107(88)90051-6.

DOI:10.1016/0027-5107(88)90051-6

PMID:2832751

Abstract

The rate of oxyradical generation by a xanthine oxidase-xanthine system to acutely cause DNA strand breakage in Chinese hamster ovary cells was studied in a phosphate-buffered saline system. DNA strand breakage, measured by a fluorometric procedure, was found to increase curvilinearly as a function of oxyradical generation. Results of studying the ability of 5 mM mannitol, 10 mM dimethylthiourea, 300 micrograms superoxide dismutase/ml, or 1 mg catalase/ml to interfere with DNA damage at a high rate of oxyradical production best supported a hydrogen peroxide-promoted mechanism for DNA breakage.

摘要

在磷酸盐缓冲盐水系统中，研究了黄嘌呤氧化酶 - 黄嘌呤系统急性导致中国仓鼠卵巢细胞DNA链断裂时的氧自由基生成速率。通过荧光测定法测量的DNA链断裂，被发现随着氧自由基生成呈曲线增加。研究5 mM甘露醇、10 mM二甲基硫脲、300微克超氧化物歧化酶/毫升或1毫克过氧化氢酶/毫升在高氧自由基产生速率下干扰DNA损伤能力的结果，最有力地支持了过氧化氢促进DNA断裂的机制。