Department of Optics and Quantum Electronics, University of Szeged, Szeged, Dóm tér 9., 6720 Hungary.
Methods Appl Fluoresc. 2017 Mar 22;5(1):017001. doi: 10.1088/2050-6120/aa6260.
A practical method has been presented for polarization sensitive localization based super-resolution microscopy using a birefringent dual wedge. The measurement of the polarization degree at the single molecule level can reveal the chemical and physical properties of the local environment of the fluorescent dye molecule and can hence provide information about the sub-diffraction sized structure of biological samples. Polarization sensitive STORM imaging of the F-Actins proved correlation between the orientation of fluorescent dipoles and the axis of the fibril.
一种实用的方法已经被提出，用于基于双折射双楔形的偏振敏感定位超分辨率显微镜。在单分子水平上测量偏振度可以揭示荧光染料分子局部环境的化学和物理性质，从而提供关于生物样品亚衍射尺寸结构的信息。F-肌动蛋白的偏振敏感 STORM 成像证明了荧光偶极子的取向与纤维轴之间的相关性。
