Salam Lateef B
Department of Biological Science, Microbiology Unit, College of Natural Sciences, Al-Hikmah University, Ilorin, Kwara, Nigeria.
3 Biotech. 2016 Jun;6(1):98. doi: 10.1007/s13205-016-0419-5. Epub 2016 Apr 8.
Two bacterial strains phylogenetically identified as Pseudomonas aeruginosa strains RM1 and SK1 displayed extensive degradation ability on waste engine oil (SAE 40W) in batch cultures. Spectrophotometric analysis revealed the presence of various heavy metals such as lead, chromium and nickel in the waste engine oil. The rate of degradation of waste engine oil by the isolates, for the first 12 days and the last 9 days were 66.3, 31.6 mg l day and 69.6, 40.0 mg l day for strains RM1 and SK1, respectively. Gas chromatographic (GC) analyses of residual waste engine oil, revealed that 66.58, 89.06 % and 63.40, 90.75 % of the initial concentration of the waste engine oil were degraded by strains RM1 and SK1 within 12 and 21 days. GC fingerprints of the waste engine oil after 12 days of incubation of strains RM1 and SK1 showed total disappearance of C, C, C, C and C hydrocarbon fractions as well as drastic reductions of C, C, C and PAHs fractions such as C-anthracene and C-pyrene. At the end of 21 days incubation, total disappearance of C-pristane, C-pyrene, one of the C-anthracene and significant reduction of C-phytane (97.2 %, strain RM1; 95.1 %, strain SK1) fractions were observed. In addition, <10 % of Day 0 values of medium fraction ranges C, and C were discernible after 21 days. This study has established the potentials of P. aeruginosa strains RM1 and SK1 in the degradation of aliphatic, aromatic and branched alkane components of waste engine oils.
两种在系统发育上被鉴定为铜绿假单胞菌菌株RM1和SK1的细菌菌株，在分批培养中对废发动机油（SAE 40W）表现出广泛的降解能力。分光光度分析表明，废发动机油中存在铅、铬和镍等各种重金属。分离菌株对废发动机油的降解速率，在前12天和最后9天，菌株RM1分别为66.3、31.6 mg·l·天，菌株SK1分别为69.6、40.0 mg·l·天。对残留废发动机油的气相色谱（GC）分析表明，在12天和21天内，菌株RM1和SK1分别降解了废发动机油初始浓度的66.58%、89.06%和63.40%、90.75%。菌株RM1和SK1培养12天后废发动机油的GC指纹图谱显示，C、C、C、C和C烃类组分完全消失，以及C、C、C和多环芳烃组分如C-蒽和C-芘大幅减少。在培养21天结束时，观察到C-姥鲛烷、C-芘、一种C-蒽完全消失，C-植烷显著减少（菌株RM1为97.2%；菌株SK1为95.1%）。此外，21天后，中等馏分范围C和C的第0天值的<10%仍可辨别。本研究确定了铜绿假单胞菌菌株RM1和SK1在降解废发动机油的脂肪族、芳香族和支链烷烃成分方面的潜力。
