Medić Ana, Lješević Marija, Inui Hideyuku, Beškoski Vladimir, Kojić Ivan, Stojanović Ksenija, Karadžić Ivanka
Department of Chemistry, Faculty of Medicine, University of Belgrade Višegradska 26 11000 Belgrade Serbia
Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade Njegoševa 12 11000 Belgrade Serbia.
RSC Adv. 2020 Apr 7;10(24):14060-14070. doi: 10.1039/c9ra10371f. eCollection 2020 Apr 6.
san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected petroleum compounds, , -alkanes (-hexadecane, -nonadecane) and polycyclic aromatic hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% and 41%, respectively, at initial concentrations of 20 mg L and in seven days. san ai showed a high biodegradative capacity on complex hydrocarbon mixtures, the aliphatic and aromatic fractions from crude oil. The efficiency of san ai degradation of crude oil fractions in seven days reached stage 3-4 of the oil biodegradation scale, which ranges from 0 (no biodegradation) to 10 (maximum biodegradation). Identified metabolites concomitant with genomic and enzymatic data indicated the terminal oxidation pathway for the -alkane degradation, and the salicylate and phthalate pathways for fluorene biodegradation. Polyextremophilic san ai, as a biosurfactant producer with multidegradative capacity for hydrocarbons, can be used in an improved strategy for environmental bioremediation of hydrocarbon-contaminated sites, including extreme habitats characterized by low or elevated temperatures, acidic or alkaline pH or high concentrations of heavy metals.
嗜碱、耐金属细菌三爱能降解选定的单一石油化合物，即正构烷烃（正十六烷、正十九烷）和多环芳烃（芴、菲、芘），在初始浓度为20 mg/L的情况下，7天内降解效率分别为80%、98%、96%、50%和41%。三爱在复杂烃类混合物（原油的脂肪族和芳香族馏分）上表现出很高的生物降解能力。三爱对原油馏分7天的降解效率达到了石油生物降解规模的3 - 4级，该规模范围从0（无生物降解）到10（最大生物降解）。与基因组和酶数据相关的已鉴定代谢物表明了正构烷烃降解的末端氧化途径，以及芴生物降解的水杨酸和邻苯二甲酸途径。多极端嗜性的三爱作为一种生物表面活性剂产生菌，具有多种烃类降解能力，可用于改进的策略，对受烃污染的场地进行环境生物修复，包括以低温或高温、酸性或碱性pH值或高浓度重金属为特征的极端生境。
