Kampmeier Tim, Arnemann Philip, Heßler Michael, Rehberg Sebastian, Morelli Andrea, Westphal Martin, Lange Matthias, Van Aken Hugo, Ertmer Christian
Department of Anaesthesiology, Intensive Care and Pain Medicine, University Hospital of Muenster, Muenster, Germany.
Department of Anaesthesiology, Intensive Care and Pain Medicine, University Hospital of Muenster, Muenster, Germany.
Vet Anaesth Analg. 2017 May;44(3):518-528. doi: 10.1016/j.vaa.2016.03.005. Epub 2017 Jan 11.
To provide physiological data and reference values in awake and anaesthetized sheep aged 6-12 months.
Descriptive study.
Data from 260 female sheep of the species Ovis orientalis aries aged 6-12 months were extracted from 10 experimental trials.
Data from pulmonary arterial thermodilution in awake (cohort 1; n = 109) and anaesthetized animals (cohort 2; n = 81), and transpulmonary thermodilution in anaesthetized animals (cohort 3; n = 70) were analysed. General anaesthesia was induced by intramuscular injection of S-ketamine and midazolam and maintained by inhaled isoflurane. Standard laboratory variables (blood gas and clinical chemistry) were assessed.
A total of 7553 single data entries from 260 healthy sheep were included. Measurement errors or invalid data documentation meant that 313 data entries (4.1%) were excluded. A small confidence interval for median values was calculated for nearly all variables. The median body weight was 39.8 kg (2.5-97.5th percentile 30.6-48.1 kg). A set of reference values (2.5-97.5th percentiles) is provided for common cardiopulmonary and laboratory variables. Compared to awake animals, haemodynamic variables were markedly influenced by anaesthesia, as reflected by a considerably lower stroke volume index in anaesthetized sheep. There were also differences in stroke volume index between the cohorts of pulmonary artery and transpulmonary thermodilution.
The present work presents a large and consistent database of a variety of physiological variables measured in healthy juvenile female sheep. The data appear to be robust and allow the establishment of standardized inclusion criteria for experimental studies and may help to better evaluate past, present and future research. Differences between pulmonary artery and transpulmonary thermodilution should be assessed in future studies.
提供6至12月龄清醒和麻醉绵羊的生理数据及参考值。
描述性研究。
从10项实验中提取了260只6至12月龄的东方绵羊种雌性绵羊的数据。
分析清醒动物（队列1；n = 109）和麻醉动物（队列2；n = 81）的肺动脉热稀释数据，以及麻醉动物（队列3；n = 70）的经肺热稀释数据。通过肌肉注射S-氯胺酮和咪达唑仑诱导全身麻醉，并用吸入异氟烷维持。评估标准实验室变量（血气和临床化学）。
共纳入了260只健康绵羊的7553条单一数据记录。测量误差或无效数据记录意味着313条数据记录（4.1%）被排除。几乎所有变量都计算了中位数的小置信区间。中位数体重为39.8千克（第2.5至97.5百分位数为30.6至48.1千克）。提供了一组常见心肺和实验室变量的参考值（第2.5至97.5百分位数）。与清醒动物相比，麻醉对血流动力学变量有显著影响，麻醉绵羊的每搏量指数明显较低就反映了这一点。肺动脉热稀释和经肺热稀释队列之间的每搏量指数也存在差异。
本研究提供了一个关于健康幼年雌性绵羊多种生理变量的庞大且一致的数据库。这些数据似乎很可靠，可为实验研究建立标准化的纳入标准，并可能有助于更好地评估过去、现在和未来的研究。未来研究应评估肺动脉热稀释和经肺热稀释之间的差异。
