Lu Yan, Li Shulin/Sl, Zhu Shiguo/Sg, Gong Yabin/Yb, Shi Jun/J, Xu Ling/L
No.2 oncology department, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, No.110, Ganhe Rd, Shanghai, China.
MD Anderson Cancer Center, the university of Texas, 1840 Old Spanish Trail, Houston, TX USA.
Biol Proced Online. 2017 Mar 17;19:2. doi: 10.1186/s12575-017-0051-8. eCollection 2017.
DNA/RNA methylation plays an important role in lung cancer initiation and progression. Liquid biopsy makes use of cells, nucleotides and proteins released from tumor cells into body fluids to help with cancer diagnosis and prognosis. Methylation of circulating tumor DNA (ctDNA) has gained increasing attention as biomarkers for lung cancer. Here we briefly introduce the biological basis and detection method of ctDNA methylation, and review various applications of methylated DNA in body fluids in lung cancer screening, diagnosis, prognosis, monitoring and treatment prediction. We also discuss the emerging role of RNA methylation as biomarkers for cancer.
DNA/RNA甲基化在肺癌的发生和发展中起着重要作用。液体活检利用肿瘤细胞释放到体液中的细胞、核苷酸和蛋白质来辅助癌症的诊断和预后评估。循环肿瘤DNA（ctDNA）的甲基化作为肺癌的生物标志物越来越受到关注。在此，我们简要介绍ctDNA甲基化的生物学基础和检测方法，并综述甲基化DNA在肺癌筛查、诊断、预后、监测及治疗预测等体液检测中的各种应用。我们还讨论了RNA甲基化作为癌症生物标志物的新作用。
