叙利亚慢性乙型肝炎患者中IFN-γ（+874 T/A）的多态性
Polymorphism of IFN-γ (+874 T/A) in Syrian patients with chronic hepatitis B.
作者信息
Al Kadi Mohamad, Monem Fawza
机构信息
Department of Clinical Biochemistry and Microbiology, Faculty of Pharmacy, Damascus University, Damascus, Syria.
Department of Clinical Biochemistry and Microbiology, Faculty of Pharmacy, Damascus University, Damascus, Syria; Clinical Laboratories Department, Al-Assad Hospital, Damascus, Syria.
出版信息
Gastroenterol Hepatol Bed Bench. 2017 Winter;10(1):34-38.
AIM
This study aimed to investigate the association of IFN- γ +874 (T/A) polymorphism with susceptibility to chronic HBV infection in the Syrian population.
BACKGROUND
Accumulating evidence indicate that the inadequate immune responses are responsible for HBV persistency. Therefore, polymorphisms in genes encoding the cytokines, which are responsible for regulation of the immune response, can affect the course and outcome of the infection. The IFN-γ +874 T/A polymorphism affects the expression of IFN-γ, which has been shown to be crucial to HBV clearance.
METHODS
In this case-control study, 140 samples were collected (70 healthy individuals, 70 chronic HBV patients), and genomic DNA was isolated. Sequencing and ARMS-PCR were performed to genotype the IFN-γ +874 T/A polymorphism.
RESULTS
Results of this study showed an association between IFN- γ +874 T/A polymorphism and the susceptibility to chronic HBV infection ( < 0.05). In addition, results showed that the AA genotype increased the risk of chronicity (OR = 3.05, 95% CI = 1.35 - 6.89), whereas the AT and TT genotypes reduced the risk of chronicity (OR = 0.33, 95% CI = 0.150 - 0.753).
CONCLUSION
Results of this study conclude that the IFN- γ +874 T/A polymorphism may be associated with the chronic HBV infection, according to the genetic model AA vs. AT&TT.
目的
本研究旨在调查叙利亚人群中IFN-γ +874（T/A）多态性与慢性乙型肝炎病毒（HBV）感染易感性之间的关联。
背景
越来越多的证据表明，免疫反应不足是HBV持续存在的原因。因此，编码负责调节免疫反应的细胞因子的基因多态性可影响感染的进程和结果。IFN-γ +874 T/A多态性影响IFN-γ的表达，已证明其对HBV清除至关重要。
方法
在这项病例对照研究中，收集了140份样本（70名健康个体，70名慢性HBV患者），并分离了基因组DNA。进行测序和ARMS-PCR以对IFN-γ +874 T/A多态性进行基因分型。
结果
本研究结果显示IFN-γ +874 T/A多态性与慢性HBV感染易感性之间存在关联（P<0.05）。此外，结果显示AA基因型增加了慢性化风险（OR = 3.05，95% CI = 1.35 - 6.89），而AT和TT基因型降低了慢性化风险（OR = 0.33，95% CI = 0.150 - 0.753）。
结论
本研究结果得出结论，根据遗传模型AA与AT&TT，IFN-γ +874 T/A多态性可能与慢性HBV感染有关。
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