Coloma F M, Niles L P
Department of Neurosciences, McMaster University, Hamilton, Ontario, Canada.
Biochem Pharmacol. 1988 Apr 1;37(7):1271-4. doi: 10.1016/0006-2952(88)90781-2.
The pineal hormone, melatonin, enhanced the sodium-independent binding of [3H]-gamma-aminobutyric acid ([3H]GABA) and [3H]muscimol in the rat cerebral cortex in vitro. This effect was augmented by preincubation of synaptic membranes with melatonin but was abolished by preincubation with Triton X-100. Saturation binding studies using [3H]GABA (2.5 to 1000 nM) indicated that the melatonin-induced enhancement of binding is due to an increase in low-affinity GABAA binding sites. These findings suggest that the central effects of melatonin involve modulation of GABAergic function.
松果体激素褪黑素增强了大鼠大脑皮层体外培养时[3H] -γ-氨基丁酸（[3H]GABA）和[3H]蝇蕈醇的非钠依赖性结合。用褪黑素预孵育突触膜可增强这种效应，但用Triton X - 100预孵育则可消除该效应。使用[3H]GABA（2.5至1000 nM）进行的饱和结合研究表明，褪黑素诱导的结合增强是由于低亲和力GABAA结合位点增加所致。这些发现表明，褪黑素的中枢效应涉及对GABA能功能的调节。
