López-Corcuera B, Giménez C, Aragón C
Departamento de Biologia Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Biochim Biophys Acta. 1988 Apr 22;939(3):467-75. doi: 10.1016/0005-2736(88)90093-4.
The effect of chronic administration of lithium salts on the lipid composition and physical properties of the synaptosomal plasma membrane was examined in rat brain. The effect of lithium treatment has been studied on the fluorescence polarization of synaptosomal plasma membrane and artificial lipid vesicles and on the lipid composition of the membranes. Fluorescence polarization of lipophilic probes was used to study membrane lipid structure. Steady-state polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH), a probe of the hydrophobic core, was significantly lower in plasma membranes from lithium-treated animals. Altered DPH polarization was due to a decrease in the order parameter of the probe. The lithium-treatment also changed the fluorescence of 1-anilino-8-naphthalene sulfonate (ANS), a probe that binds to the polar head group of the phospholipids and to proteins on the membrane surface. Synaptic plasma membranes from treated rats presented no significant changes on the cholesterol-to-phospholipid ratio, although the phospholipid class distribution was altered and the membrane phospholipid unsaturation increased. In summary, the neural plasma membranes became disorder after chronic lithium administration at therapeutic levels. This structural change may be due to changes in plasma membrane phospholipid distribution and to the degree of unsaturation of phospholipid fatty acids.
在大鼠脑中研究了长期给予锂盐对突触体细胞膜脂质组成和物理性质的影响。研究了锂处理对突触体细胞膜和人工脂质囊泡的荧光偏振以及膜脂质组成的影响。使用亲脂性探针的荧光偏振来研究膜脂质结构。作为疏水核心探针的1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）的稳态偏振在锂处理动物的质膜中显著降低。DPH偏振的改变是由于探针的序参数降低。锂处理还改变了1 - 苯胺基 - 8 - 萘磺酸盐（ANS）的荧光，ANS是一种与磷脂的极性头部基团和膜表面蛋白质结合的探针。尽管磷脂类分布发生改变且膜磷脂不饱和度增加，但处理大鼠的突触质膜在胆固醇与磷脂的比率上没有显著变化。总之，在治疗水平长期给予锂后，神经质膜变得无序。这种结构变化可能是由于质膜磷脂分布的改变以及磷脂脂肪酸的不饱和度程度所致。
