Kálmán M, Kardos J
First Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary.
Int J Neurosci. 1988 Jan;38(1-2):45-51. doi: 10.3109/00207458809000481.
In earlier studies we have established that rat cortical synaptosomes isolated after a 2-h postmortem storage period showed functional performance and morphological characteristics similar to those isolated immediately after decapitation. The present paper is an account of further functional studies involving the activity of Na-K- and Mg-dependent ATPase and the binding of ouabain in postmortem synaptosomes. These functions were found to survive without major alteration even in the 6-h postmortem fractions. On the other hand the 6-h postmortem fractions showed a very slow oxygen consumption unaffected by DNP and veratrine. In both control and postmortem synaptosomes the actual activity of Na-K-ATPase as calculated from the ouabain-sensitive component of oxygen consumption was consistently less than the potential activity of the enzyme revealed if determination was carried out on the basis of phosphate splitting.
在早期研究中我们已证实，死后储存2小时后分离的大鼠皮质突触体，其功能表现和形态特征与断头后立即分离的突触体相似。本文是关于死后突触体中钠钾和镁依赖性ATP酶活性及哇巴因结合的进一步功能研究报告。即使在死后6小时的组分中，这些功能也能基本保持不变。另一方面，死后6小时的组分显示出非常缓慢的氧气消耗，且不受二硝基苯酚（DNP）和藜芦碱的影响。在对照和死后突触体中，根据氧气消耗对哇巴因敏感的组分计算出的钠钾ATP酶的实际活性，始终低于基于磷酸盐裂解测定所显示的该酶的潜在活性。
