Wang Lin, Yan Ting, Yang Lanping, Zhang Kexia, Jia Jingming
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning 110016, China.
Heilongjiang Provincial Institute for Food and Drug Control, 711 Wanggang Street, Harbin, Heilongjiang 150077, China.
J Chromatogr Sci. 2017 May 1;55(5):528-535. doi: 10.1093/chromsci/bmx005.
In the present study, a high performance liquid chromatography (HPLC) method was developed and validated for fingerprint profiling to evaluate the quality consistency of the Da Hong Pao tea. The chromatographic separation was performed on a USA Waters C18 column (4.6 mm × 250 mm, 5 μm, Waters-XBridge Shield RP 18) with gradient elution. The column temperature was maintained at 30°C and the detection wavelength was set at 278 nm. The results demonstrated that the developed HPLC method achieved the desired linearity, precision, accuracy and limit of quantification. The fingerprints were developed and evaluated qualitatively using the ratio quantitative fingerprint method. The fingerprint analysis of the reference standards (caffeine, catechin, epicatechin, epigallocatechin, epicatechingallate, Epigallocatechingallate, theaflavin, theaflavin-3-gallate, theaflavin-3'-gallate and theaflavin-3, 3'-gallate) was found to reflect the content of the reference standards in the tea samples. Furthermore, the fingerprint-efficacy relationship among the components as well as the antioxidant activity in vitro were investigated. The selected samples showed good antioxidant activity based on the results. Our study demonstrated that the method of HPLC fingerprint profiling combine the antioxidant activity assay offering a reliable and efficient approach to quantitatively evaluate the quality consistency of the traditional Chinese medicine and other herbal preparations.
在本研究中，开发并验证了一种高效液相色谱（HPLC）方法用于指纹图谱分析，以评估大红袍茶的质量一致性。色谱分离在一台美国沃特世C18柱（4.6 mm×250 mm，5μm，沃特世XBridge Shield RP 18）上进行，采用梯度洗脱。柱温保持在30℃，检测波长设定为278 nm。结果表明，所开发的HPLC方法达到了所需的线性、精密度、准确度和定量限。采用比值定量指纹图谱法对指纹图谱进行定性开发和评价。对参考标准品（咖啡因、儿茶素、表儿茶素、表没食子儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯、茶黄素、茶黄素-3-没食子酸酯、茶黄素-3'-没食子酸酯和茶黄素-3,3'-没食子酸酯）的指纹图谱分析发现，其反映了茶叶样品中参考标准品的含量。此外，还研究了各成分之间的指纹图谱-药效关系以及体外抗氧化活性。根据结果，所选样品显示出良好的抗氧化活性。我们的研究表明，HPLC指纹图谱分析方法结合抗氧化活性测定，为定量评价中药及其他草药制剂的质量一致性提供了一种可靠且高效的方法。
