Islam Johirul, Zaman Kamaruz, Chakrabarti Srijita, Sharma Bora Nilutpal, Mandal Santa, Pratim Pathak Manash, Srinivas Raju Pakalapati, Chattopadhyay Pronobesh
Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India.
Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam 786004, India.
J Chromatogr Sci. 2017 Jul 1;55(6):645-653. doi: 10.1093/chromsci/bmx021.
A simple, accurate and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the estimation of ethyl 2-aminobenzoate (EAB) in a matrix type monolithic polymeric device and validated as per the International Conference on Harmonization guidelines. The analysis was performed isocratically on a ZORBAX Eclipse plus C18 analytical column (250 × 4.4 mm, 5 μm) and a diode array detector (DAD) using acetonitrile and water (75:25 v/v) as the mobile phase by keeping the flow-rate constant at 1.0 mL/min. Determination of EAB was not interfered in the presence of excipients. Inter- and intra-day relative standard deviations were not higher than 2%. Mean recovery was between 98.7 and 101.3%. Calibration curve was linear in the concentration range of 0.5-10 µg/mL. Limits of detection and quantification were 0.19 and 0.60 µg/mL, respectively. Thus, the present report put forward a novel method for the estimation of EAB, an emerging insect repellent, by using RP-HPLC technique.
已开发出一种简单、准确且灵敏的反相高效液相色谱（RP-HPLC）方法，用于测定基质型整体聚合物装置中的2-氨基苯甲酸乙酯（EAB），并根据国际协调会议指南进行了验证。分析在ZORBAX Eclipse plus C18分析柱（250×4.4 mm，5μm）上进行等度洗脱，使用二极管阵列检测器（DAD），以乙腈和水（75:25 v/v）作为流动相，流速保持恒定在1.0 mL/min。在辅料存在的情况下，EAB的测定不受干扰。日间和日内相对标准偏差均不高于2%。平均回收率在98.7%至101.3%之间。校准曲线在0.5-10 µg/mL的浓度范围内呈线性。检测限和定量限分别为0.19和0.60 µg/mL。因此，本报告提出了一种使用RP-HPLC技术测定新型驱虫剂EAB的新方法。
