用于检测小鼠肝炎病毒血清抗体的Track XI荧光免疫测定法与Bio-EnzaBead酶联免疫吸附测定法的比较。
Comparison of Track XI fluorometric immunoassay with Bio-EnzaBead enzyme-linked immunosorbent assay for detection of serum antibody to mouse hepatitis virus.
作者信息
La Regina M C, Lonigro J, Woods L, Hall W C, Doyle R E
机构信息
Department of Comparative Medicine, St. Louis University Medical School, Missouri 63104.
出版信息
J Clin Microbiol. 1988 Mar;26(3):573-5. doi: 10.1128/jcm.26.3.573-575.1988.
The Track XI system (Microbiological Associates, Bethesda, Md.) was compared with the Bio-EnzaBead assay (Organon Teknika, Durham, N.C.) for the detection of antibody to mouse hepatitis virus (MHV). Strain A/J mice were inoculated intranasally with MHV type 3. Sera were collected at 1, 2, 4, and 9 weeks postinoculation and tested. Individual serum samples were retested twice by each method. The results suggested that the Track XI system was more sensitive and reliable than the Bio-EnzaBead assay in detecting antibody to MHV type 3 in individual serum samples from A/J mice.
将轨道XI系统（微生物学联合公司，马里兰州贝塞斯达）与生物酶珠分析法（奥加农技术公司，北卡罗来纳州达勒姆）进行比较，以检测小鼠肝炎病毒（MHV）抗体。用3型MHV经鼻接种A/J品系小鼠。在接种后1、2、4和9周收集血清并进行检测。每个血清样本通过每种方法重新检测两次。结果表明，在检测A/J小鼠个体血清样本中3型MHV抗体方面，轨道XI系统比生物酶珠分析法更灵敏、更可靠。
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