Nica Ionela Cristina, Stan Miruna Silvia, Dinischiotu Anca, Popa Marcela, Chifiriuc Mariana Carmen, Lazar Veronica, Pircalabioru Gratiela G, Bezirtzoglou Eugenia, Iordache Ovidiu G, Varzaru Elena, Dumitrescu Iuliana, Feder Marcel, Vasiliu Florin, Mercioniu Ionel, Diamandescu Lucian
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania.
Department of Botanic-Microbiology, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 60101 Bucharest, Romania.
Nanomaterials (Basel). 2016 Nov 15;6(11):214. doi: 10.3390/nano6110214.
The development of innovative technologies to modify natural textiles holds an important impact for medical applications, including the prevention of contamination with microorganisms, particularly in the hospital environment. In our study, Fe and N co-doped TiO₂ nanoparticles have been obtained via the hydrothermal route, at moderate temperature, followed by short thermal annealing at 400 °C. These particles were used to impregnate polyester (PES) materials which have been evaluated for their morphology, photocatalytic performance, antimicrobial activity against bacterial reference strains, and in vitro biocompatibility on human skin fibroblasts. Microscopic examination and quantitative assays have been used to evaluate the cellular morphology and viability, cell membrane integrity, and inflammatory response. All treated PES materials specifically inhibited the growth of Gram-negative bacilli strains after 15 min of contact, being particularly active against . PES fabrics treated with photocatalysts did not affect cell membrane integrity nor induce inflammatory processes, proving good biocompatibility. These results demonstrate that the treatment of PES materials with TiO₂-1% Fe-N particles could provide novel biocompatible fabrics with short term protection against microbial colonization, demonstrating their potential for the development of innovative textiles that could be used in biomedical applications for preventing patients' accidental contamination with microorganisms from the hospital environment.
开发用于改性天然纺织品的创新技术对医学应用具有重要影响，包括预防微生物污染，尤其是在医院环境中。在我们的研究中，通过水热法在适中温度下制备了铁和氮共掺杂的二氧化钛纳米颗粒，随后在400℃下进行短时间热退火。这些颗粒用于浸渍聚酯（PES）材料，并对其形态、光催化性能、对细菌参考菌株的抗菌活性以及对人皮肤成纤维细胞的体外生物相容性进行了评估。通过显微镜检查和定量分析来评估细胞形态和活力、细胞膜完整性以及炎症反应。所有经处理的PES材料在接触15分钟后均能特异性抑制革兰氏阴性杆菌菌株的生长，对[此处原文缺失具体菌株名称]尤其有效。用光催化剂处理的PES织物不影响细胞膜完整性，也不引发炎症过程，证明具有良好的生物相容性。这些结果表明，用1%铁氮共掺杂的二氧化钛颗粒处理PES材料可以提供具有短期抗微生物定植保护作用的新型生物相容性织物，证明了它们在开发可用于生物医学应用以防止患者意外受到医院环境中微生物污染的创新纺织品方面的潜力。
