LeVay S
Salk Institute for Biological Studies, San Diego, California 92138.
J Comp Neurol. 1988 Mar 8;269(2):265-74. doi: 10.1002/cne.902690210.
Small injections of peroxidase-labeled wheat germ agglutinin into cat area 18 gave rise to patches of labeled cells and axon terminals around the injection site. In EM sections it was found that the labeled cells had a pattern of synaptic inputs characteristic of spiny-dendrite neurons (pyramidal or spiny stellate cells). The labeled axon terminals formed type 1 (asymmetric) synapses, most of which were made onto dendritic spines. In other experiments injections of fluorescent beads were made into area 18, giving rise to a similar patchy distribution of labeled cells. The sections were then processed for immunocytochemical demonstration of gamma-aminobutyric acid (GABA). The bead-labeled cells in the patches were GABA negative. The findings suggest that the patchy projections mediate mutual excitation between groups of spiny-dendrite neurons.
向猫的18区小剂量注射过氧化物酶标记的小麦胚芽凝集素，在注射部位周围产生了标记细胞和轴突终末的斑块。在电子显微镜切片中发现，标记细胞具有棘状树突神经元（锥体神经元或棘状星形细胞）特有的突触输入模式。标记的轴突终末形成1型（不对称）突触，其中大多数与树突棘形成突触。在其他实验中，将荧光珠注射到18区，产生了类似的标记细胞斑块状分布。然后对切片进行免疫细胞化学处理以显示γ-氨基丁酸（GABA）。斑块中珠标记的细胞GABA呈阴性。这些发现表明，斑块状投射介导了棘状树突神经元群之间的相互兴奋。
