a Perm State National Research University , Perm , Russia.
b Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences , Perm , Russia.
Infect Dis (Lond). 2017 Aug;49(8):594-600. doi: 10.1080/23744235.2017.1306101. Epub 2017 Mar 23.
The goal of our study was to compare the following two methods of assessment of pertussis post-vaccination immunity: bacterial agglutination test and pertussis toxin enzyme-linked immunosorbent assay (ELISA).
The study was carried out in Perm Region, Russia. We measured pertussis immunity using two serological methods: ELISA of IgG to pertussis toxin (PT) and the agglutination test (AT) among 135 children, in the age range from 2 months to 17 years old. The immunization schedule included four doses of DTwP: at 3, 4.5 and 6 months of age and a booster at 18 months. All participants were divided into six age groups.
The percentage of samples with IgG level less than the detection limit in vaccinated children was 52.2%. The total seropositivity rate (the percent of children with agglutinin titres ≥1:160) in vaccinated children was 47.8%. Only a weak association was observed between agglutinin and anti-PT IgG titres (R = .3). Neither the primary nor the booster vaccination with DTwP influenced the IgG levels in children. Agglutinin titres significantly increased after vaccination and declined 5 years after the booster dose. Significant growth of IgG concentration was observed in 11-year-olds, indicating the presence of B. pertussis circulation in the childhood population.
Based on the obtained results and the results of other authors, we summarize that anti-PT ELISA should be carefully used to assess the population immunity to pertussis. Currently, there is neither a serological test that accurately determines the protection against pertussis nor a distinctive criterion of protection that can be applied in seroepidemiological studies.
我们的研究目的是比较以下两种评估百日咳疫苗接种后免疫的方法：细菌凝集试验和百日咳毒素酶联免疫吸附试验（ELISA）。
该研究在俄罗斯彼尔姆地区进行。我们使用两种血清学方法测量百日咳免疫力：针对百日咳毒素（PT）的 IgG 的 ELISA 和凝集试验（AT），研究对象为 135 名年龄在 2 个月至 17 岁的儿童。免疫接种计划包括四剂 DTwP：3、4.5 和 6 个月龄时各一剂，18 个月龄时一剂加强针。所有参与者分为六个年龄组。
在接种疫苗的儿童中，IgG 水平低于检测限的样本百分比为 52.2%。接种疫苗的儿童总血清阳性率（抗体滴度≥1:160 的儿童百分比）为 47.8%。仅观察到凝集素和抗-PT IgG 滴度之间存在微弱关联（R=0.3）。DTwP 的初次接种或加强接种均未影响儿童的 IgG 水平。接种后，凝集素滴度显著增加，加强针接种 5 年后下降。在 11 岁儿童中观察到 IgG 浓度显著增长，表明儿童人群中存在百日咳杆菌的流行。
根据获得的结果和其他作者的结果，我们总结认为，抗-PT ELISA 应谨慎用于评估人群对百日咳的免疫力。目前，既没有一种能够准确确定对百日咳保护作用的血清学检测方法，也没有一种可用于血清流行病学研究的独特保护标准。
