转化的大鼠和小鼠细胞中缺失起始点的猿猴病毒40 DNA的多次插入和串联重复。
Multiple insertions and tandem repeats of origin-minus simian virus 40 DNA in transformed rat and mouse cells.
作者信息
Blanck G, Li D, Pomert E, Pollack R, Chen S
机构信息
Department of Biological Sciences, Columbia University, New York, New York 10027.
出版信息
J Virol. 1988 May;62(5):1520-3. doi: 10.1128/JVI.62.5.1520-1523.1988.
Stable simian virus 40 (SV40) transformation requires integration and expression of the early region of the SV40 genome. We have examined the amount and state of integrated viral DNA of SV40-transformed NIH 3T3 mouse and F2408 rat fibroblast lines generated by transfection with either wild-type or origin-defective SV40 DNA. A functional SV40 replication origin was not required for multiple inserts and partial-repeat structures to form in NIH 3T3 mouse transformants. In contrast, partial repeats in F2408 rat transformants were rare when the SV40 replication origin was intact and not detected at all when it was defective.
稳定的猿猴病毒40（SV40）转化需要SV40基因组早期区域的整合和表达。我们检测了用野生型或起源缺陷型SV40 DNA转染产生的SV40转化的NIH 3T3小鼠和F2408大鼠成纤维细胞系中整合病毒DNA的量和状态。在NIH 3T3小鼠转化体中形成多个插入片段和部分重复结构不需要功能性的SV40复制起点。相比之下，当SV40复制起点完整时，F2408大鼠转化体中的部分重复很少见，而当它有缺陷时则完全检测不到。
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