Robins D M, Ripley S, Henderson A S, Axel R
Cell. 1981 Jan;23(1):29-39. doi: 10.1016/0092-8674(81)90267-1.
A series of rat liver cotransformed cell lines have been constructed containing from 5 to 100 copies of a variant human growth hormone gene. We have used hybridization in situ to demonstrate that most, if not all, cotransformed sequences reside in a chromosome of the host cell. In each of four cell lines examined, hybridization was restricted to a single chromosomal site with no extrachromosomal sites apparent. The site was invariant within each line; however, each line revealed a different site of integration for transforming sequences. In two of the four lines, transforming DNA resided at or near the site of gross chromosomal rearrangements, in one line near an rDNA site, and in one line in the middle of an apparently normal chromosome. Thus, insertion is not restricted to a unique chromosome or chromosomal region.
已经构建了一系列大鼠肝脏共转化细胞系，这些细胞系含有5到100个拷贝的变异人生长激素基因。我们利用原位杂交证明，即使不是全部，大多数共转化序列都位于宿主细胞的一条染色体上。在检测的四个细胞系中，每个细胞系的杂交都局限于一个单一的染色体位点，没有明显的染色体外位点。该位点在每个细胞系内是不变的；然而，每个细胞系都显示出转化序列整合的不同位点。在这四个细胞系中的两个细胞系中，转化DNA位于染色体大规模重排位点处或附近，在一个细胞系中靠近rDNA位点，在一个细胞系中位于一条明显正常的染色体中间。因此，插入并不局限于某一条独特的染色体或染色体区域。
