用于埃及鸡传染性支气管炎病毒感染（IBV）最新分子诊断的新型实时和传统逆转录聚合酶链反应，该病毒感染常与禽流感和新城疫病毒共同感染有关。
New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.
作者信息
Naguib Mahmoud M, El-Kady Magdy F, Lüschow Dörte, Hassan Kareem E, Arafa Abdel-Satar, El-Zanaty Ali, Hassan Mohamed K, Hafez Hafez M, Grund Christian, Harder Timm C
机构信息
The Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Suedufer 10, Greifswald Insel-Riems 17493, Germany; National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, P.O. Box 246, Giza 12618, Egypt.
Poultry Disease Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
出版信息
J Virol Methods. 2017 Jul;245:19-27. doi: 10.1016/j.jviromet.2017.02.018. Epub 2017 Mar 21.
In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.
在埃及，目前有两种地理分布受限的传染性支气管炎冠状病毒（IBV）基因型正在传播，对家禽业造成不利影响。开发了一种针对IBV核衣壳基因（N）的灵敏实时RT-PCR检测方法，用于筛查临床样本中是否存在IBV。还开发了扩增IBV S1基因高变区（HVRs 1-2和3）的常规RT-PCR方法，并将扩增产物直接用于对埃及鸡临床样本中的IBV田间毒株进行基于核苷酸序列的分型。
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