Sun Der-Shan, Tseng Yao-Hsuan, Wu Wen-Shiang, Wong Ming-Show, Chang Hsin-Hou
Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien 97004, Taiwan.
Nanobiomedical Research Center, Tzu-Chi University, Hualien 97004, Taiwan.
Nanomaterials (Basel). 2016 Dec 28;7(1):2. doi: 10.3390/nano7010002.
Conventional photocatalysts are primarily stimulated using ultraviolet (UV) light to elicit reactive oxygen species and have wide applications in environmental and energy fields, including self-cleaning surfaces and sterilization. Because UV illumination is hazardous to humans, visible light-responsive photocatalysts (VLRPs) were discovered and are now applied to increase photocatalysis. However, fundamental questions regarding the ability of VLRPs to trigger DNA mutations and the mutation types it elicits remain elusive. Here, through plasmid transformation and β-galactosidase α-complementation analyses, we observed that visible light-responsive platinum-containing titania (TiO₂) nanoparticle (NP)-mediated photocatalysis considerably reduces the number of transformants. This suggests that such photocatalytic reactions cause DNA damage. DNA sequencing results demonstrated that the DNA damage comprises three mutation types, namely nucleotide insertion, deletion and substitution; this is the first study to report the types of mutations occurring after photocatalysis by TiO₂-VLRPs. Our results may facilitate the development and appropriate use of new-generation TiO₂ NPs for biomedical applications.
传统的光催化剂主要通过紫外线（UV）光激发以产生活性氧物种，并在环境和能源领域有着广泛应用，包括自清洁表面和杀菌。由于紫外线照射对人体有害，因此发现了可见光响应型光催化剂（VLRP），目前已将其应用于增强光催化作用。然而，关于VLRP引发DNA突变的能力及其引发的突变类型等基本问题仍然难以捉摸。在这里，通过质粒转化和β-半乳糖苷酶α-互补分析，我们观察到可见光响应型含铂二氧化钛（TiO₂）纳米颗粒（NP）介导的光催化作用显著减少了转化子的数量。这表明这种光催化反应会导致DNA损伤。DNA测序结果表明，DNA损伤包括三种突变类型，即核苷酸插入、缺失和替换；这是第一项报道TiO₂-VLRP光催化后发生的突变类型的研究。我们的结果可能有助于新一代TiO₂纳米颗粒在生物医学应用中的开发和合理使用。
