Belogurov A A, Efimova E P, Del'ver E P, Zavil'gel'skiĭ G B
Mol Gen Mikrobiol Virusol. 1987 Dec(12):34-40.
2-aminopurine (2-AP) and 5-bromouracil, strong mutagens of base analog type, were found to induce efficiently the alleviation of I type restriction in Escherichia coli. 2-AP induced restriction alleviation occurs in recA, lexA and mut- mutants, but no additional relief of restriction is registered in dam-bacteria in the presence of sublethal 2-AP concentrations. 2-AP specifically alleviates I type restriction in Escherichia coli (EcoA, EcoB, EcoD, and EcoK) and does not affect restriction systems of II (EcoRI) and III (EcoP1) types. We suggest that 2-AP-induced mismatches and other replication errors may be signals inducing restriction alleviation in Escherichia coli.
2-氨基嘌呤（2-AP）和5-溴尿嘧啶是碱基类似物类型的强诱变剂，它们被发现能有效诱导大肠杆菌中I型限制作用的缓解。2-AP诱导的限制作用缓解发生在recA、lexA和mut-突变体中，但在亚致死浓度的2-AP存在下，dam-细菌中未观察到额外的限制作用缓解。2-AP特异性地缓解大肠杆菌（EcoA、EcoB、EcoD和EcoK）中的I型限制作用，而不影响II型（EcoRI）和III型（EcoP1）限制系统。我们认为，2-AP诱导的错配和其他复制错误可能是诱导大肠杆菌中限制作用缓解的信号。
