Shi Quan, Qian Zhiyong, Liu Donghua, Sun Jie, Xu Juan, Guo Ximin
Department of Stomatology, Chinese People's Liberation Army General HospitalBeijing, China; Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical SciencesBeijing, China.
Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical SciencesBeijing, China; School of Biological Science and Medical Engineering, Beihang UniversityBeijing, China.
Front Physiol. 2017 Mar 8;8:143. doi: 10.3389/fphys.2017.00143. eCollection 2017.
Mesenchymal stem cells (MSCs) play a critical role in cartilage tissue engineering. However, MSCs-derived chondrocytes or cartilage tissues are not stable and easily lose the cellular and cartilage phenotype during long-term culture or implantation . As a result, chondrocytes phenotypic instability can contribute to accelerated ossification. Thus, it is a big challenge to maintain their correct phenotype for engineering hyaline cartilage. As one member of the natriuretic peptide family, C-type natriuretic peptide (CNP) is found to correlate with the development of the cartilage, affect the chondrocytes proliferation and differentiation. Besides, based on its biological effects on protection of extracellular matrix of cartilage and inhibition of mineralization, we hypothesize that CNP may contribute to the stability of chondrocyte phenotype of MSCs-derived chondrocytes.
间充质干细胞（MSCs）在软骨组织工程中发挥着关键作用。然而，MSCs来源的软骨细胞或软骨组织并不稳定，在长期培养或植入过程中容易丧失细胞和软骨表型。因此，软骨细胞表型不稳定性会导致骨化加速。因此，对于工程化透明软骨而言，维持其正确表型是一项巨大挑战。作为利钠肽家族的一员，C型利钠肽（CNP）被发现与软骨发育相关，影响软骨细胞的增殖和分化。此外，基于其对软骨细胞外基质的保护和矿化抑制的生物学效应，我们推测CNP可能有助于MSCs来源软骨细胞的软骨细胞表型稳定性。
