微小RNA-15a通过直接靶向……抑制肝癌细胞的迁移和侵袭。
MiR-15a suppresses hepatocarcinoma cell migration and invasion by directly targeting .
作者信息
Liu Bo, Sun Ting, Wu Gang, Shang-Guan Hui, Jiang Zhao-Jin, Zhang Ji-Ren, Zheng Yan-Fang
机构信息
Oncology Center, Zhujiang Hospital of Southern Medical University Guangzhou 510282, Guangdong, China.
Department of Oncology, Foshan Hospital, Southern Medical University Foshan 528000, Guangdong, China.
出版信息
Am J Transl Res. 2017 Feb 15;9(2):520-532. eCollection 2017.
Abstract
PURPOSE
This study aimed to determine the function of miR-15a in HCC, and identify as a target of miR-15a.
METHODS
RNA expression was evaluated by quantitative real-time PCR (qRT-PCR). The effects of miR-15a or on HCC cells were evaluated by transwell migration assay and western blot analysis. , the predicted target, has been frequently verified by luciferase assay.
RESULTS
MiR-15a was markedly downregulated in sphere culture HCC cells by qRT-PCR. was predicted to be a potential target of miR-15a using bioinformatics analysis. This prediction has been frequently verified by luciferase assay and western blot. A positive correlation between and the migration ability of HCC cells was demonstrated by transwell assays. MiR-15a mimic suppressed expression to weaken HCC cell migration ability. On the other hand, miR-15a inhibitor upregulated and induced HCC cell migration.
CONCLUSION
MiR-15a could suppress HCC progression through the repression of , making miR-15a a potential therapeutic target.
摘要
目的
本研究旨在确定miR-15a在肝癌中的作用，并鉴定 为miR-15a的一个靶点。
方法
通过定量实时聚合酶链反应（qRT-PCR）评估RNA表达。通过Transwell迁移试验和蛋白质免疫印迹分析评估miR-15a或 对肝癌细胞的影响。通过荧光素酶报告基因检测频繁验证预测的靶点 。
结果
通过qRT-PCR检测发现，miR-15a在球形培养的肝癌细胞中显著下调。使用生物信息学分析预测 是miR-15a的潜在靶点。这一预测已通过荧光素酶报告基因检测和蛋白质免疫印迹频繁验证。Transwell试验证明 与肝癌细胞的迁移能力呈正相关。miR-15a模拟物抑制 表达以削弱肝癌细胞迁移能力。另一方面，miR-15a抑制剂上调 并诱导肝癌细胞迁移。
结论
miR-15a可通过抑制 来抑制肝癌进展，使miR-15a成为潜在的治疗靶点。
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