Cao Shiguang, Sun Ruiying, Wang Wei, Meng Xia, Zhang Yuping, Zhang Na, Yang Shuanying
Department of Respiratory, The Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an, China; Department of Nuclear Medicine, The Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an, China.
Department of Respiratory, The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an, China.
Am J Transl Res. 2017 Feb 15;9(2):674-682. eCollection 2017.
RNA helicase DHX9 is a member of human RNA enzymes. Previous studies have reported that DHX9 is highly expressed in various types of malignant tumor. However, its role in the progression of lung cancer remains to be fully clarified. The present study aims to investigate the oncogenic role of DHX9 in serum, tissues and lung cancer cell lines in vitro. We used RNA interference to downregulate DHX9 expression in A549 cells using a small interfering RNA lentiviral vector. Subsequently, enoxacin was used to inhibit cell proliferation, and this effect was detected using MTT. The results showed that DHX9 was overexpressed in the serum and tissues of lung cancer, especially in small cell lung cancer. Though enoxacin suppressed the proliferation of NSCLC cells, the inhibition effect was diminished when DHX9 was knocked down. In conclusion, the present study provided evidence suggesting that DHX9 was overexpressed in lung cancer and may contribute to the growth of lung cancer, and enoxacin may inhibit the proliferation based on DHX9. Thus DHX9 may be used as a diagnostic marker and a potential therapeutic target for the treatment of NSCLC.
RNA解旋酶DHX9是人类RNA酶的成员之一。先前的研究报道，DHX9在各种类型的恶性肿瘤中高表达。然而，其在肺癌进展中的作用仍有待充分阐明。本研究旨在探讨DHX9在血清、组织及体外肺癌细胞系中的致癌作用。我们使用RNA干扰技术，通过小干扰RNA慢病毒载体下调A549细胞中DHX9的表达。随后，使用依诺沙星抑制细胞增殖，并通过MTT检测此效应。结果显示，DHX9在肺癌血清和组织中过表达，尤其是在小细胞肺癌中。虽然依诺沙星抑制了非小细胞肺癌细胞的增殖，但当DHX9被敲低时，抑制作用减弱。总之，本研究提供的证据表明，DHX9在肺癌中过表达，可能促进肺癌生长，且依诺沙星可能基于DHX9抑制增殖。因此，DHX9可作为非小细胞肺癌的诊断标志物和潜在治疗靶点。
