Analysis of and Expression in Gastric Cancer and Their Possible Correlation with Tumor Clinicopathological Factors.

PURPOSE

This study aimed to analyze and expression profiles in patients with gastric cancer, and examine the possible relationship between the expressions of each gene and clinicopathological factors.

MATERIALS AND METHODS

Expression of these genes in formalin-fixed paraffin embedded (FFPE) tissues, collected from 40 patients with gastric cancer and 40 healthy controls, was analyzed. Differences in gene expression among patient and normal samples were identified using the GraphPad Prism 5 software. For the analysis of real-time polymerase chain reaction products, GelQuantNET software was used.

RESULTS

Our findings demonstrated that both and mRNA expression levels were downregulated in gastric cancer samples compared with those in the normal controls. No significant relationship was found between the expression of these genes and gender (P-value, 0.4835 vs. 0.6350), but there were significant changes associated with age (P-value, 0.0004 vs. 0.0001) and stage of disease (P-value, 0.0019 vs. 0.0001). In addition, there was a direct relationship between gene expression and metastasis (P-value, 0.0462), in contrast to that did not demonstrate any significant correlation (P-value, 0.1833).

CONCLUSIONS

The results suggest that expression profiling of and can be used for diagnosis of gastric cancer, and specifically, gene could be considered as a biomarker for the detection of gastric cancer progression.