肠道隐窝类器官：肠道干细胞的分离、体外培养及光学观察
Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation.
作者信息
Chen Yun, Li Chuan, Tsai Ya-Hui, Tseng Sheng-Hong
机构信息
Department of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei, Taiwan.
Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan, Taiwan.
出版信息
Methods Mol Biol. 2019;1576:215-228. doi: 10.1007/7651_2017_21.
The isolation and culture of intestinal stem cells (ISCs) was first demonstrated in the very recent decade with the identification of ISC marker Lgr5. The growth of ISCs into crypt organoids provides an in vitro model for studying the mucosal physiology, intestinal cancer tumorigenesis, and intestinal regeneration. Here, we describe two different isolation protocols and demonstrate a fixation method that aids in the confocal observation of the organoids.
肠道干细胞（ISC）的分离和培养在最近十年才首次通过ISC标志物Lgr5的鉴定得以证实。ISC生长为隐窝类器官为研究黏膜生理学、肠道癌肿瘤发生和肠道再生提供了一个体外模型。在此，我们描述两种不同的分离方案，并展示一种有助于类器官共聚焦观察的固定方法。
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