Rajak Jyoti, Bawaskar Manisha, Rathod Dnyaneshwar, Agarkar Gauravi, Nagaonkar Dipali, Gade Aniket, Rai Mahendra
Department of Biotechnology, Ramniranjan Jhunjhunwala College, Ghatkopar, Mumbai.
Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, Maharashtra, India.
J Sci Food Agric. 2017 Oct;97(13):4562-4570. doi: 10.1002/jsfa.8324. Epub 2017 Apr 18.
In recent years, agro-bionanotechnology has paved the way towards revolutionizing current practices in the food and agricultural fields. In the present study, the effect of copper nanoparticles (CuNPs) alone and in combination with the growth-promoting fungus Piriformospora indica on 2-week-old seedlings of Cajanus cajan was evaluated. Gelatin-coated stable CuNPs were synthesized by the chemical reduction method using hydrazine hydrate as a reducing agent.
CuNPs were characterized by using UV-visible spectroscopy, zeta potential measurement and transmission electron microscopy. The nanoparticles were found to be quite spherical with a diameter within the range 40 ± 10 nm. After the application of CuNPs and P. indica to the host plant C. cajan, the vitality of plants was determined using a Handy-PEA (plant efficiency analyzer) instrument. Handy-PEA analysis (which measures chlorophyll a fluorescence) indicated that seedlings inoculated with a combination of CuNPs and P. indica were the healthiest and also showed maximum vitality as compared to seedlings inoculated with CuNPs or P. indica alone.
These results suggest that CuNPs in combination with P. indica can serve as a nanobiofertilizer for enhancement of the growth and productivity of C. cajan. © 2017 Society of Chemical Industry.
近年来，农业生物纳米技术为变革食品和农业领域的现有实践铺平了道路。在本研究中，评估了单独的铜纳米颗粒（CuNPs）以及与促生长真菌印度梨形孢组合对木豆两周龄幼苗的影响。使用水合肼作为还原剂，通过化学还原法合成了明胶包覆的稳定CuNPs。
通过紫外可见光谱、zeta电位测量和透射电子显微镜对CuNPs进行了表征。发现纳米颗粒呈相当规则的球形，直径在40±10nm范围内。将CuNPs和印度梨形孢应用于宿主植物木豆后，使用Handy-PEA（植物效率分析仪）仪器测定植物的活力。Handy-PEA分析（测量叶绿素a荧光）表明，与单独接种CuNPs或印度梨形孢的幼苗相比，接种CuNPs和印度梨形孢组合的幼苗最健康，活力也最高。
这些结果表明，CuNPs与印度梨形孢组合可作为纳米生物肥料，用于提高木豆的生长和生产力。©2017化学工业协会。
