Garcia-Nuñez Marian, Marti Sara, Puig Carmen, Perez-Brocal Vicente, Millares Laura, Santos Salud, Ardanuy Carmen, Moya Andres, Liñares Josefina, Monsó Eduard
CIBER de Enfermedades Respiratorias (CIBERes), Instituto de Salud Carlos III, Madrid, Spain E-28029.
Department of Respiratory Medicine, Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí I3PT, Sabadell, Spain E-08208.
Future Microbiol. 2017 Apr;12:379-392. doi: 10.2217/fmb-2016-0127. Epub 2017 Mar 24.
The bronchial microbiome of severe chronic obstructive pulmonary disease patients colonized by Pseudomonas aeruginosa was analyzed using 16S rRNA gene sequencing to identify differences related to biofilm-forming capacity.
PATIENTS & METHODS: Patient sputum samples from 21 patients were studied.
Statistically significant differences related to biofilm-forming capacity were only found for genera with relative abundances <1%, and Fusobacterium was over-represented when biofilm-forming capacity was high. Genera with relative abundances >50% which increased from baseline were observed in 10/14 exacerbations, but corresponded to Pseudomonas only in three episodes, while other pathogenic genera were identified in seven.
The bronchial microbiome shows differences according with P. aeruginosa biofilm-forming capacity. Pathogenic microorganisms other than P. aeruginosa cause a significant part of the exacerbations in colonized chronic obstructive pulmonary disease patients.
运用16S rRNA基因测序分析被铜绿假单胞菌定植的重度慢性阻塞性肺疾病患者的支气管微生物群，以确定与生物膜形成能力相关的差异。
研究了21例患者的痰液样本。
仅在相对丰度<1%的属中发现了与生物膜形成能力相关的统计学显著差异，当生物膜形成能力较高时，梭杆菌属的占比过高。在10/14次病情加重中观察到相对丰度>50%且较基线水平增加的属，但仅在3次发作中对应于铜绿假单胞菌，而在7次发作中鉴定出了其他致病属。
支气管微生物群因铜绿假单胞菌的生物膜形成能力而表现出差异。在定植的慢性阻塞性肺疾病患者中，除铜绿假单胞菌外的致病微生物导致了相当一部分病情加重。
