酵母细胞壁产品（CitriStim®）对实验性球虫病攻毒期间蛋鸡和肉鸡免疫应答及盲肠微生物区系的影响。

Effect of whole yeast cell product supplementation (CitriStim®) on immune responses and cecal microflora species in pullet and layer chickens during an experimental coccidial challenge.

机构信息

Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster, OH 44691.

ADM Animal Nutrition, Quincy, IL 62301.

出版信息

Poult Sci. 2017 Jul 1;96(7):2049-2056. doi: 10.3382/ps/pew482.

DOI:10.3382/ps/pew482

PMID:28339714

Abstract

Three separate experiments were conducted to study the effects of whole yeast cell product supplementation in pullets and layer hens. Body weight gain, fecal and intestinal coccidial oocyst counts, cecal microflora species, cytokine mRNA amounts, and CD4+ and CD8+ T-cell populations in the cecal tonsils were analyzed following an experimental coccidial infection. In Experiment I, day-old Leghorn layer chicks were fed 3 experimental diets with 0, 0.1, or 0.2% whole yeast cell product (CitriStim®, ADM, Decatur, IL). At 21 d of age, birds were challenged with 1 × 105 live coccidial oocysts. Supplementation with whole yeast cell product decreased the fecal coccidial oocyst count at 7 (P = 0.05) and 8 (P < 0.01) d post-challenge. In Experiment II, 27-week old Leghorn layer hens were fed 3 experimental diets with 0, 0.05 or 0.1% whole yeast cell product and challenged with 1 × 105 live coccidial oocysts on d 25 of whole yeast cell product feeding. Supplementation with whole yeast cell product decreased the coccidial oocyst count in the intestinal content (P < 0.01) at 5, 13, and 38 d post-coccidial challenge. Supplementation with whole yeast cell product increased relative proportion of Lactobacillus (P < 0.01) in the cecal tonsils 13 d post-coccidial challenge. Supplementation with whole yeast cell product decreased CD8+ T cell percentages (P < 0.05) in the cecal tonsils at 5 d post-coccidial challenge. In Experiment III, 32-week-old Leghorn layer hens were fed 3 experimental diets with 0, 0.1, or 0.2% whole yeast cell product and challenged with 1 × 105 live coccidial oocysts on d 66 of whole yeast cell product feeding. At 5 d post-coccidial challenge, whole yeast cell product supplementation down-regulated (P = 0.01) IL-10 mRNA amount. It could be concluded that supplementing whole yeast cell product can help minimize coccidial infection in both growing pullets and layer chickens.

摘要

进行了三项独立的实验，以研究在育雏鸡和蛋鸡中添加完整酵母细胞产品对其的影响。在实验性球虫感染后，分析体重增加、粪便和肠道球虫卵囊计数、盲肠微生物种类、细胞因子 mRNA 量以及盲肠扁桃体中的 CD4+和 CD8+T 细胞群体。在实验 I 中，给 1 日龄的来航层鸡喂食 3 种含有 0、0.1 或 0.2%完整酵母细胞产品（CitriStim ®，ADM，迪凯特，IL）的实验日粮。在 21 日龄时，鸡群用 1×105 活球虫卵囊进行攻毒。补充完整的酵母细胞产品可降低攻毒后 7 天（P=0.05）和 8 天（P<0.01）的粪便球虫卵囊计数。在实验 II 中，27 周龄来航层鸡喂食 3 种含有 0、0.05 或 0.1%完整酵母细胞产品的实验日粮，并在整个酵母细胞产品喂养的第 25 天用 1×105 活球虫卵囊攻毒。补充完整的酵母细胞产品降低了肠道内容物中的球虫卵囊计数（P<0.01），在球虫攻毒后 5、13 和 38 天。补充完整的酵母细胞产品增加了盲肠扁桃体中乳杆菌的相对比例（P<0.01），在球虫攻毒后 13 天。补充完整的酵母细胞产品降低了盲肠扁桃体中 CD8+T 细胞的百分比（P<0.05），在球虫攻毒后 5 天。在实验 III 中，32 周龄来航层鸡喂食 3 种含有 0、0.1 或 0.2%完整酵母细胞产品的实验日粮，并在整个酵母细胞产品喂养的第 66 天用 1×105 活球虫卵囊攻毒。在球虫攻毒后 5 天，补充完整的酵母细胞产品下调了 IL-10 mRNA 量（P=0.01）。可以得出结论，补充完整的酵母细胞产品可以帮助减少生长育雏鸡和蛋鸡的球虫感染。