Omara Islam I, Pender Chasity M, White Mallory B, Dalloul Rami A
Avian Immunobiology Laboratory, Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, USA.
Animal and Poultry Division, Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Animals (Basel). 2021 Jan 12;11(1):159. doi: 10.3390/ani11010159.
This study investigated the effects of a yeast-derived β-glucan (Auxoferm YGT) supplementation on mRNA expression of immune response genes in the spleen, thymus, and bursa of broiler chickens during a mixed infection. Day (d)-old chicks ( = 1440) were fed diets containing 0% or 0.1% YGT. On d 8 post-hatch, half the replicate pens ( = 8) were challenged with a mixed inoculum of , , and . On d 10 and d 14 post-hatch, the spleen, thymus, and bursa were collected to evaluate mRNA abundance by quantitative real-time PCR. Data were analyzed using PROC GLIMMIX model (2-way interaction) and differences were established by LS-MEANS with significance reported at ≤ 0.05. In spleen tissues at d 10, expression of interleukin (IL)-10 and inducible nitric oxide synthase (iNOS) were elevated in both 0.1% YGT-fed challenged and non-challenged birds. In thymus tissues at d 14, expression of IL-10, IL-17F, interferon (IFN)-γ, iNOS, and macrophage migration inhibitory factor (MIF) were elevated in challenged birds fed 0.1% YGT. In bursal tissues at d 10 and d 14, expression of IL-10, IFN-γ, iNOS (d 10 only), and MIF were elevated in 0.1% YGT-fed challenged and non-challenged birds. Dietary β-glucan supplementation to chicken diets modulated their immune response to the challenge.
本研究调查了酵母来源的β-葡聚糖（Auxoferm YGT）添加物对肉鸡在混合感染期间脾脏、胸腺和法氏囊中免疫反应基因mRNA表达的影响。1日龄雏鸡（n = 1440）被饲喂含有0%或0.1% YGT的日粮。在孵化后第8天，一半重复栏（n = 8）用大肠杆菌、沙门氏菌和产气荚膜梭菌的混合接种物进行攻毒。在孵化后第10天和第14天，采集脾脏、胸腺和法氏囊，通过定量实时PCR评估mRNA丰度。数据使用PROC GLIMMIX模型（双向交互作用）进行分析，并通过最小二乘均值确定差异，显著性以P≤0.05报告。在第10天的脾脏组织中，饲喂0.1% YGT的攻毒和未攻毒鸡中白细胞介素（IL）-10和诱导型一氧化氮合酶（iNOS）的表达均升高。在第14天的胸腺组织中，饲喂0.1% YGT的攻毒鸡中IL-10、IL-17F、干扰素（IFN）-γ、iNOS和巨噬细胞迁移抑制因子（MIF）的表达升高。在第10天和第14天的法氏囊组织中，饲喂0.1% YGT的攻毒和未攻毒鸡中IL-10、IFN-γ、iNOS（仅第10天）和MIF的表达升高。在鸡日粮中添加β-葡聚糖可调节其对攻毒的免疫反应。
