Jiang L, Yang X Y, Li X Y, Deng P K, Jing G X, Wang X Q, Xing Z, Zhao R G
School of Agronomy, Jilin Agriculture University, Changchun, Jilin Province, China.
School of Agronomy, Jilin Agriculture University, Changchun, Jilin Province, China
Genet Mol Res. 2017 Mar 22;16(1):gmr-16-01-gmr.16019173. doi: 10.4238/gmr16019173.
Doubling method is the technical barriers in maize haploid breeding. It was very important to establish the independent intellectual property rights for doubling method. In this experiment, the maize haploid inducer, TG15, was used for producing maternal haploids. Also, haploids were obtained from two kinds of maternal genotypes involved in the experiment, including high-oil type and common type. Significant differences were observed among offspring of various genotypes in the recovery of haploid fertilization. In 21 hybrid offspring haploids, the average powder rate was 8.28%, and the seed setting rate was 4.98%. The experimental results showed that when the hybrids were treated with 0.08% colchicine, the average powder rate and seed setting rate of offspring haploids were 35.53 and 20.30%, respectively, which were significantly higher than the hybrids with natural recovery ability. This study primarily established the doubling method of haploids called "bud seedling method" in China which was very practicably in maize doubled haploid breeding.
加倍方法是玉米单倍体育种中的技术瓶颈。建立具有自主知识产权的加倍方法至关重要。本试验以玉米单倍体诱导系TG15为母本诱导产生单倍体，供试母本包括高油型和普通型两种基因型。不同基因型后代单倍体受精恢复率存在显著差异。在21个杂交后代单倍体中，平均散粉率为8.28%，结实率为4.98%。试验结果表明，用0.08%秋水仙素处理杂交种后，后代单倍体平均散粉率和结实率分别为35.53%和20.30%，显著高于具有自然恢复能力的杂交种。本研究初步建立了适合我国玉米双单倍体育种实际应用的单倍体加倍方法——“芽苗法”。
