Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France.
UMR-S 1172, JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, University of Lille, Lille 59000, France; INSERM, UMR-S 1172, Laboratory of Development and Plasticity of the Neuroendocrine Brain, Lille 59000, France.
Cell. 2017 Mar 23;169(1):161-173.e12. doi: 10.1016/j.cell.2017.03.008.
Generating a precise cellular and molecular cartography of the human embryo is essential to our understanding of the mechanisms of organogenesis in normal and pathological conditions. Here, we have combined whole-mount immunostaining, 3DISCO clearing, and light-sheet imaging to start building a 3D cellular map of the human development during the first trimester of gestation. We provide high-resolution 3D images of the developing peripheral nervous, muscular, vascular, cardiopulmonary, and urogenital systems. We found that the adult-like pattern of skin innervation is established before the end of the first trimester, showing important intra- and inter-individual variations in nerve branches. We also present evidence for a differential vascularization of the male and female genital tracts concomitant with sex determination. This work paves the way for a cellular and molecular reference atlas of human cells, which will be of paramount importance to understanding human development in health and disease. PAPERCLIP.
生成精确的人类胚胎细胞和分子图谱对于我们理解正常和病理条件下器官发生的机制至关重要。在这里，我们将全胚胎免疫染色、3DISCO 透明化和光片成像相结合，开始构建人类妊娠前三个月发育的三维细胞图谱。我们提供了发育中的周围神经系统、肌肉系统、血管系统、心肺系统和泌尿生殖系统的高分辨率 3D 图像。我们发现，成人样的皮肤神经支配模式在第一个三个月结束前就已经建立，显示出神经分支在个体内和个体间的重要差异。我们还提供了证据表明，男性和女性生殖道的血管生成存在差异，同时伴随着性别决定。这项工作为人类细胞的细胞和分子参考图谱铺平了道路，这对于理解健康和疾病状态下的人类发育至关重要。
