Long-term stability and nutrient removal efficiency of aerobic granules at low organic loads.

The feasibility of application of aerobic granular sludge cultivated with high organic loads for biological nutrient removal (BNR) from low-strength wastewater was studied. Granules obtained with high-strength (COD=1400mg/L) wastewater were fed with medium (COD=700mg/L) and then low-strength (COD=400mg/L) wastewater. The granules rapidly acclimated to the medium-strength wastewater. However, feeding with low-strength wastewater reduced the F/M ratio from 0.4 to 0.2gCOD/gVSSd and granules disintegration occurred. Re-granulation was obtained after poor settling biomass was washed out and the F/M ratio reached 0.4gCOD/gVSSd. Disintegration of granules coincided with the decrease in extracellular polymeric substances (EPS) content and protein-to-carbohydrate ratio and re-granulation was assisted with the increase in EPS and protein-to-carbohydrate ratio. The results indicated that cultivation of aerobic granules with high organic loads and its implication for BNR treatment of low-strength wastewater while balancing the F/M ratio can be an alternative to reduce start-up period.