Penagos Dayana Grisales, Victoria Jenny Rodríguez, Manrique Mateo Villarraga
ECCA Group, School of Engineering, Universidad del Valle, Santiago de Cali, Colombia.
MethodsX. 2022 Apr 22;9:101710. doi: 10.1016/j.mex.2022.101710. eCollection 2022.
This paper proposes and develops a protocol for measuring the aerobic granulation potential of sludge, aiming to provide an affordable and simple alternative that can facilitate the development of aerobic granulation technology. In this sense, the protocol comprises a set of parameters and considerations that interact to create a controlled environment and stimulate cell population clustering. All of this is done in the context of procedural simplicity, low cost, and the speed at which results are obtained. The protocol is essentially a three-stage method: preparation of the substrate, adaptation of the inoculum, and implementation of the protocol. Simple parameters were measured to evaluate the granulation process: SVI, settling velocity, and morphological parameters. The protocol was validated according to optimal ranges and criteria previously established in the literature. For this purpose, an activated sludge inoculum from a domestic wastewater treatment plant was submitted to the protocol, obtaining an optimal response of the biomass (SVI =13.90 mL g, settling velocity= 25,79 m h, Diameter > 0.2 mm) in a relatively short time (7 d). The results show that this protocol can constitute a tool for evaluation and decision-making using traditional laboratory equipment and is applicable at different scales.
本文提出并开发了一种用于测量污泥好氧颗粒化潜力的方案，旨在提供一种经济实惠且简单的替代方法，以促进好氧颗粒化技术的发展。从这个意义上讲，该方案包括一组相互作用的参数和注意事项，以创建一个可控环境并刺激细胞群体聚集。所有这些都是在操作简单、成本低以及获得结果的速度快的背景下完成的。该方案本质上是一种三阶段方法：底物制备、接种物驯化和方案实施。通过测量简单的参数来评估颗粒化过程：污泥体积指数（SVI）、沉降速度和形态参数。该方案根据文献中先前确定的最佳范围和标准进行了验证。为此，将来自生活污水处理厂的活性污泥接种物应用于该方案，在相对较短的时间（7天）内获得了生物量的最佳响应（SVI = 13.90 mL/g，沉降速度 = 25.79 m/h，直径 > 0.2 mm）。结果表明，该方案可以构成一种使用传统实验室设备进行评估和决策的工具，并且适用于不同规模。
