Landy-Otsuka F, Scheffler I E
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5001-5. doi: 10.1073/pnas.75.10.5001.
We have investigated the induction of ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) activity in a temperature-sensitive cell cycle mutant of Chinese hamster fibroblasts. This activity is not induced at the nonpermissive temperature, although the synthesis of the majority of proteins is normal. From a combination of studies with inhibitors of mRNA synthesis and maturation (alpha-amanitin, and cordycepin) and of proteins synthesis (cycloheximide, diphtheria toxin, and emetine), we conclude that the temperature-sensitive block is at the level of translation of one or more specific mRNAs.
我们研究了中国仓鼠成纤维细胞温度敏感型细胞周期突变体中鸟氨酸脱羧酶（L-鸟氨酸羧基裂解酶，EC 4.1.1.17）活性的诱导情况。尽管大多数蛋白质的合成正常，但在非允许温度下该活性未被诱导。通过结合使用mRNA合成和成熟抑制剂（α-鹅膏蕈碱和放线菌素）以及蛋白质合成抑制剂（环己酰亚胺、白喉毒素和依米丁）进行研究，我们得出结论，温度敏感型阻断发生在一种或多种特定mRNA的翻译水平。
