用于纯化5-羟基二十碳四烯酸脱氢酶的亲和层析配体的设计与合成
Design and synthesis of affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase.
作者信息
Nagendra Reddy Chintam, Ye Qiuji, Patel Pranav, Sivendran Sashikala, Chourey Shishir, Wang Rui, Anumolu Jaganmohan R, Grant Gail E, Powell William S, Rokach Joshua
机构信息
Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne FL 32901, USA.
Meakins-Christie Laboratories, Centre for Translational Biology, McGill University Hospital Centre Research Institute, 1001 Decarie Blvd, Montreal, QC H4A 3J1, Canada.
出版信息
Bioorg Med Chem. 2017 Jan 1;25(1):116-125. doi: 10.1016/j.bmc.2016.10.017. Epub 2016 Oct 15.
Arachidonic acid (AA) is converted to biologically active metabolites by different pathways, one of the most important of which is initiated by 5-lipoxygenase (5-LO). 5-Hydroxyeicosatetraenoic acid (5-HETE), although possessing only weak biological activity itself, is oxidized to 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), a potent chemoattractant for eosinophils and neutrophils. Our main goal is to determine how the biosynthesis of 5-oxo-ETE is regulated and to determine its pathophysiological roles. To achieve this task, we designed and synthesized affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase (5-HEDH), the enzyme responsible for the formation of 5-oxo-ETE.
花生四烯酸（AA）通过不同途径转化为生物活性代谢产物，其中最重要的途径之一由5-脂氧合酶（5-LO）启动。5-羟基二十碳四烯酸（5-HETE）虽然自身仅具有微弱的生物活性，但会被氧化为5-氧代-6,8,11,14-二十碳四烯酸（5-氧代-ETE），这是一种对嗜酸性粒细胞和中性粒细胞具有强大趋化作用的物质。我们的主要目标是确定5-氧代-ETE的生物合成是如何被调控的，并确定其病理生理作用。为完成这项任务，我们设计并合成了用于纯化5-羟基类花生酸脱氢酶（5-HEDH）的亲和层析配体，该酶负责5-氧代-ETE的形成。
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