Pfreundschuh M, Shiku H, Takahashi T, Ueda R, Ransohoff J, Oettgen H F, Old L J
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5122-6. doi: 10.1073/pnas.75.10.5122.
Sera from 30 patients with astrocytoma were tested for antibody reacting with cell surface antigens of cultured autologous astrocytoma cells. Ten percent of the patients had antibody detectable by mixed hemadsorption assays, approximately 50% by immune adherence and protein A assays, and 100% by anti-C3-mixed hemadsorption assays. Absorption analysis of reactive sera with autologous, allogeneic, and xenogeneic cells permitted the definition of three classes of astrocytoma cell surface antigens. Class I antigens showed an absolute restriction to autologous astrocytoma cells. Class II antigens were shared by all astrocytomas tested and could be detected also on neuroblastoma, sarcoma, and some (but not all) melanoma cell lines; these antigens were not found on cell lines derived from carcinomas or normal tissues. Class III antigens were widely distributed on cultured normal and malignant cells of human and animal origin. In this series, sera from 2 patients recognized class I antigens, 4 patients' serum recognized class II antigens, and 13 patients' sera recognized class III antigens. Absorption tests have shown that the AJ (class II) antigen of astrocytoma is serologically related to the previously described AH (class II) antigen of melanoma; in tests of nine melanoma cell lines, there was a correspondence between the AJ and AH phenotypes. This method of autologous typing provides a way to classify the cell surface antigens of astrocytomas and to assess the clinical significance of humoral immunity to these antigens.
对30例星形细胞瘤患者的血清进行检测，以寻找与培养的自体星形细胞瘤细胞表面抗原发生反应的抗体。10%的患者通过混合血细胞吸附试验可检测到抗体，通过免疫黏附和蛋白A试验约为50%，通过抗C3 -混合血细胞吸附试验则为100%。用自体、同种异体和异种细胞对反应性血清进行吸收分析，确定了三类星形细胞瘤细胞表面抗原。I类抗原绝对局限于自体星形细胞瘤细胞。II类抗原在所有检测的星形细胞瘤中均有表达，在神经母细胞瘤、肉瘤和一些（但不是所有）黑色素瘤细胞系中也能检测到；在癌或正常组织来源的细胞系中未发现这些抗原。III类抗原广泛分布于人和动物来源的培养正常细胞和恶性细胞上。在该系列中，2例患者的血清识别I类抗原，4例患者的血清识别II类抗原，13例患者的血清识别III类抗原。吸收试验表明，星形细胞瘤的AJ（II类）抗原与先前描述的黑色素瘤的AH（II类）抗原在血清学上相关；在对9个黑色素瘤细胞系的检测中，AJ和AH表型之间存在对应关系。这种自体分型方法为星形细胞瘤细胞表面抗原的分类以及评估针对这些抗原的体液免疫的临床意义提供了一种途径。
