Zhang Xinye, Yang Qin, Rucker Elizabeth, Thomason Wade, Balint-Kurti Peter
Maize Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, China.
Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA.
Theor Appl Genet. 2017 Jun;130(6):1285-1295. doi: 10.1007/s00122-017-2888-2. Epub 2017 Mar 24.
In this study we mapped the QTL Qgls8 for gray leaf spot (GLS) resistance in maize to a ~130 kb region on chromosome 8 including five predicted genes. In previous work, using near isogenic line (NIL) populations in which segments of the teosinte (Zea mays ssp. parviglumis) genome had been introgressed into the background of the maize line B73, we had identified a QTL on chromosome 8, here called Qgls8, for gray leaf spot (GLS) resistance. We identified alternate teosinte alleles at this QTL, one conferring increased GLS resistance and one increased susceptibility relative to the B73 allele. Using segregating populations derived from NIL parents carrying these contrasting alleles, we were able to delimit the QTL region to a ~130 kb (based on the B73 genome) which encompassed five predicted genes.
在本研究中，我们将玉米抗灰斑病（GLS）的数量性状基因座Qgls8定位到8号染色体上一个约130 kb的区域，该区域包含5个预测基因。在之前的研究中，我们利用近等基因系（NIL）群体，将大刍草（Zea mays ssp. parviglumis）基因组片段导入玉米自交系B73的背景中，在8号染色体上鉴定出一个数量性状基因座，即这里所说的Qgls8，其与抗灰斑病（GLS）有关。我们在这个数量性状基因座上鉴定出了不同的大刍草等位基因，其中一个相对于B73等位基因能增强对灰斑病的抗性，另一个则增加了易感性。利用携带这些对比等位基因的NIL亲本衍生的分离群体，我们能够将数量性状基因座区域界定到一个约130 kb（基于B73基因组）的范围，该区域包含5个预测基因。
