Spontaneous and ACTH-induced interrenal steroidogenesis in juvenile coho salmon (Oncorhynchus kisutch): effects of monovalent ions and osmolality in vitro.

We determined the in vitro effects of changes in extracellular monovalent ion levels and osmotic pressure on the spontaneous and adrenocorticotropin (ACTH)-stimulated interrenal activity of coho salmon (Oncorhynchus kisutch). We used a perifusion system of incubation and monitored interrenal activity by measuring the effluent cortisol content with a radioimmunoassay. An increase in the medium osmolality with mannitol, from 206 to 290 or 353 mosmol, caused an increase in the spontaneous release of cortisol only slightly (compared with the much greater increase induced by porcine-ACTH). A similar minor increase was observed when NaCl was elevated from 130 to 180 mM. On the other hand, the spontaneous release of cortisol was not affected by increasing the KCl level from 3.2 to 9.6 mM, but was clearly increased when KCl was raised from 3.2 mM to a supraphysiological level of 27.2 mM. Ionic or osmolality changes, within the physiological range observed in coho salmon plasma, did not affect the characteristics of interrenal secretion of cortisol in response to porcine-ACTH. If our results with interrenal cells in vitro are representative of the basic functioning of the cells in vivo, then one would have to conclude that changes in concentrations of plasma monovalent ions or in osmotic pressure may not play a significant physiological role in the regulation of interrenal steroidogenesis or corticosteroid release in coho salmon.