Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970, Brazil.
Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970, Brazil.
J Dairy Sci. 2017 Jun;100(6):4287-4289. doi: 10.3168/jds.2016-11922. Epub 2017 Mar 23.
Corynebacterium bovis is a mastitis-causing microorganism responsible for economic losses related to decrease in milk production. The aim of the study was identify Corynebacterium spp. strains recovered from milk samples of subclinical mastitis by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Samples were collected during a 10-mo mastitis-monitoring program in a high-production dairy farm. In this study, 80 strains were analyzed; from these 54 (67.5%) were identified at species level as Corynebacterium bovis, 24 (31.2%) isolates were identified at the genus level as Corynebacterium spp., and only 1 (1.35%) isolated had unreliable identification. Results demonstrated that MALDI-MS could be an important technique for the identification of Corynebacterium spp. in milk.
牛分枝杆菌是一种引起乳腺炎的微生物，会导致与牛奶产量下降相关的经济损失。本研究旨在使用基质辅助激光解吸电离质谱（MALDI-MS）鉴定从亚临床乳腺炎牛奶样本中回收的棒状杆菌属菌株。在高生产力奶牛场的乳腺炎监测计划期间收集了 10 个月的样本。在这项研究中，分析了 80 株，其中 54 株（67.5%）在种水平上被鉴定为牛分枝杆菌，24 株（31.2%）分离株在属水平上被鉴定为棒状杆菌属，只有 1 株（1.35%）分离株的鉴定不可靠。结果表明，MALDI-MS 可能是一种用于鉴定牛奶中棒状杆菌属的重要技术。
Zotero 插件