Beijing You'an Hospital, Capital Medical University, Beijing, 100069, China.
Beijing immunotech applied science limited.
Int J Infect Dis. 2017 Jun;59:37-43. doi: 10.1016/j.ijid.2017.03.015. Epub 2017 Mar 22.
As antiretroviral therapy provides long term viral suppression but no cure, alternative therapies such as adoptive cellular therapy have thus been investigated in the anti-AIDS field.
This study sought to establish a HLA-A02 specific CTL cell culture method with comparison of the effects of different cytokines used in CTL cultivation to decide the best cultivation environment. In order to produce CTLs with targeted HLA-A02 restricted antigen specificity for adoptive cellular therapy, we evaluated autologous PBMC cultivation in different cytokine environment to select a better expansion condition to produce qualified CTL production.
We co-cultivated PBMC and peptides of these patients with HLA-A02 allele with different cytokines. After cultivation, multiple parameters were tested.
This model of CTL cultivation is successful in redirecting the specificity of antigen recognition and safely for HLA-A02+ patients cell adoptive therapy.
由于抗逆转录病毒疗法可提供长期的病毒抑制，但不能治愈，因此在抗艾滋病领域已经研究了替代疗法，如过继性细胞疗法。
本研究旨在建立一种 HLA-A02 特异性 CTL 细胞培养方法，并比较 CTL 培养中使用的不同细胞因子的效果，以确定最佳的培养环境。为了产生针对 HLA-A02 限制性抗原特异性的、用于过继性细胞治疗的 CTL，我们评估了在不同细胞因子环境中自体 PBMC 的培养，以选择更好的扩增条件来产生合格的 CTL 产物。
我们用不同的细胞因子共同培养 PBMC 和具有 HLA-A02 等位基因的患者肽。培养后，测试了多个参数。
1）单独的细胞因子 IL-2 可以有效地扩增 HLA-A02 特异性 CTL 细胞，并且整个过程中的 CTL 计数均>85%。2）培养结束时的 HLA-A02 特异性细胞主要是 CD3+CD8+细胞。3）干扰素刺激试验表明，扩增的 CTL 分泌的 IFN-γ比培养前更多（0.9%-11.70%）。
该 CTL 培养模型成功地改变了抗原识别的特异性，并且对 HLA-A02+患者的细胞过继治疗是安全的。
