Omer C A, Stein D, Cohen S N
Department of Genetics, Stanford University School of Medicine, California 94305.
J Bacteriol. 1988 May;170(5):2174-84. doi: 10.1128/jb.170.5.2174-2184.1988.
We report that transformation of Streptomyces lividans with cloned DNA of the SLP1 genetic element results in integration of the element at the same chromosomal locus (attB) normally occupied by SLP1 in its original host, Streptomyces coelicolor, and in S. lividans that has received SLP1 by mating. We constructed SLP1 derivatives that can integrate foreign DNA at the attB site and used these to introduce adventitious DNA sequences into the S. lividans chromosome. We also identified three regions of SLP1 essential for its integration and demonstrated that integration of the SLP1 element does not require expression of functions necessary for stable maintenance or transfer of extrachromosomal forms of SLP1.
我们报道，用SLP1遗传元件的克隆DNA转化变铅青链霉菌，会导致该元件整合到与在其原始宿主天蓝色链霉菌以及通过接合获得SLP1的变铅青链霉菌中SLP1正常占据的相同染色体位点（attB）。我们构建了能够在attB位点整合外源DNA的SLP1衍生物，并利用这些衍生物将外来DNA序列引入变铅青链霉菌染色体。我们还鉴定出了SLP1整合所必需的三个区域，并证明SLP1元件的整合不需要表达对于SLP1染色体外形式的稳定维持或转移所必需的功能。
